In the Gulf of Maine, the copepod Calanus finmarchicus co-occurs with the neurotoxin-producing dinoflagellate, Alexandrium fundyense. The copepod is resistant to this toxic alga, but little is known about other effects. Gene expression profiles were used to investigate the physiological response of females feeding for two and five days on a control diet or a diet containing either a low or a high dose of A. fundyense. The physiological responses to the two experimental diets were similar, but changed between the time points. At 5-days the response was characterized by down-regulated genes involved in energy metabolism. Detoxification was not a major component of the response. Instead, genes involved in digestion were consistently regulated, suggesting that food assimilation may have been affected. Thus, predicted increases in the frequency of blooms of A. fundyense could affect C. finmarchicus populations by changing the individuals’ energy budget and reducing their ability to build lipid reserves.
2dayCvsLD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the low dose (LD) treatment of Alexandrium fundyense for 2 days compared with the control diet Rhodomonas spp.. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
2dayCvsHD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the high dose (HD) treatment of Alexandrium fundyense for 2 days compared with the control diet Rhodomonas spp.. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
5dayCvsLD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the low dose (LD) treatment of Alexandrium fundyense for 5 days compared with the control diet Rhodomonas spp.. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
5dayCvsHD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the high dose (HD) treatment of Alexandrium fundyense for5 days compared with the control diet Rhodomonas spp.. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
2dayLDvsHD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the low dose (LD) treatment of Alexandrium fundyense for 2 days compared with the high dose (HD) treatment. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
5dayLvsHD
List of up- and down-regulated genes in Calanus finmarchicus adult females feeding on the low dose (LD) treatment of Alexandrium fundyense for 5 days compared with the high dose (HD) treatment. For each gene NCBI Accession No., fold change (absolute) and annotation (blastx) are listed.
CfinmarchicusReferenceTranscriptome
Annotation list for Calanus finmarchicus reference transcriptome consisting of a total of 38,104 transcripts (Lenz et al., 2014). For each transcript NCBI Accession No., sequence length (bp) and annotation results are reported. Annotation results from blastx against Non-redundant (NR) protein database (BLAST2GO) consist of: sequence description, hit description, hit accession No., e-value, similarity score, alignment length and positives. In bold transcripts that resulted annotated using BLAST2GO, but were also in silico annotated using the workflow described in Christie et al., 2013a; Christie et al., 2013b; Christie et al., 2014. In red bolded, transcripts that did not retrieve annotation using BLAST2GO, but were in silico annotated as described in Christie et al., 2013a; Christie et al., 2013b; Christie et al., 2014. For bolded transcripts (red and black) the assigned name and reference paper are listed. For more information on the transcriptome refer to Lenz et al., 2014.