Data from: Assessing early fitness consequences of exotic gene flow in the wild: a field study with Iberian pine relicts

Unger GM, Heuertz M, Vendramin GG, Robledo-Arnuncio JJ

Date Published: September 29, 2015

DOI: http://dx.doi.org/10.5061/dryad.13bd2

 

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Title Nuclear and chloroplast microsatellite genotypes of open-pollinated seeds and naturally established seedlings in Pinus pinaster at Fuencaliente (Ciudad Real, Spain)
Downloaded 3 times
Description One line per combined nuclear-chloroplast individual genotype, comprising 12 nSSR diploid loci and 6 cpSSR haploid regions. For nSSR, the two alleles at each locus are listed in consecutive columns. Missing data coded with zeros. Samples collected in the field. DNA extracted with Invisorb DNA Plant HTS 96 Kit. Electrophoresis of amplified products with LI-COR 4300 DNA Analyzer for cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied Biosystems) for nSSRs. Fragment size determination through visual inspection using external size markers and reference samples with known allele size for cpSSR, and internal size standard and GeneMapper software (Applied Biosystems) for nSSR. The reference adult genotypes used in the study are available at http://dx.doi.org/10.5061/dryad.pq6hg.
Download Ppinaster_Fuencaliente_cp&nSSRs.csv (117.8 Kb)
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Title Nuclear and chloroplast microsatellite genotypes of open-pollinated seeds and naturally established seedlings in Pinus sylvestris at Sierra Nevada (Granada, Spain)
Downloaded 3 times
Description One line per combined nuclear-chloroplast individual genotype, comprising 10 nSSR diploid loci and 7 cpSSR haploid regions. For nSSR, the two alleles at each locus are listed in consecutive columns. Missing data coded with zeros. Samples collected in the field. DNA extracted with Invisorb DNA Plant HTS 96 Kit. Electrophoresis of amplified products with LI-COR 4300 DNA Analyzer for cpSSRs, and 3130xL and 3500 Genetic Analyzers (Applied Biosystems) for nSSRs. Fragment size determination through visual inspection using external size markers and reference samples with known allele size for cpSSR, and internal size standard and GeneMapper software (Applied Biosystems) for nSSR. The reference adult genotypes used in the study are available at http://dx.doi.org/10.5061/dryad.pq6hg
Download Psylvestris_SierraNevada_cp&nSSRs.csv (85.91 Kb)
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When using this data, please cite the original publication:

Unger GM, Heuertz M, Vendramin GG, Robledo-Arnuncio JJ (2016) Assessing early fitness consequences of exotic gene flow in the wild: a field study with Iberian pine relicts. Evolutionary Applications, 9(2):367-380. http://dx.doi.org/10.1111/eva.12333

Additionally, please cite the Dryad data package:

Unger GM, Heuertz M, Vendramin GG, Robledo-Arnuncio JJ (2015) Data from: Assessing early fitness consequences of exotic gene flow in the wild: a field study with Iberian pine relicts. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.13bd2
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