Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.

 

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Title FishPeakArea
Downloaded 33 times
Description Peak area calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.
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Title FishPeakHeight
Downloaded 30 times
Description Peak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for European minnow samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.
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Title RicePeakArea
Downloaded 26 times
Description Peak area calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.
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Title RicePeakHeight
Downloaded 27 times
Description Peak height in Relative Fluorescent Units (RFU) calculated by Genemapper from ABI sequencer chromatograms for rice samples. Each column labelled "Peak X bp" is an AFLP locus with a fragment length of X base pairs. In the column "Polymerase", "PHU" refers to Phusion, "HOT" to Phusion Hot Start II, and "TAQ" to standard Taq polymerase.
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When using this data, please cite the original publication:

Brelsford A, Collin H, Perrin N, Fumagalli L (2011) Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers. Molecular Ecology Resources 12(1): 123-127. http://dx.doi.org/10.1111/j.1755-0998.2011.03063.x

Additionally, please cite the Dryad data package:

Brelsford A, Collin H, Perrin N, Fumagalli L (2011) Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.1hg75
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