Data from: Selection experiments reveal trade-offs between swimming and twitching motilities in Pseudomonas aeruginosa

Taylor TB, Buckling A

Date Published: May 31, 2011

DOI: http://dx.doi.org/10.5061/dryad.230t0

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title Cross Inoculation Data
Downloaded 31 times
Description Cross Inoculation Data.csv, Data description: Data used to create Figure 1: Cross inoculation of evolved strains between evolutionary environments. The data gives the area of dispersal of the evolved strains relative to the ancestral strain from which all strains were derived. There are 6 replicate lines, which were averaged to give “Mean Area Relative to Ancestor”. Data was collected in the lab and analysed using ImageJ. Columns correspond to: Strain Replicate: the name allocated to each of the replicate lines, e.g. H1 corresponds to replicate line 1 evolved on hard agar. Agar Type on which Strain was Evolved: Strains were either evolved in hard or liquid agar. Agar Type onto which Strain was Inoculated: Strains were either inoculated onto hard or liquid agar. Area Relative to Ancestor: Area of evolved strain under each treatment group was divided by area of ancestral strain under the same treatment to give a relative measurement of area dispersed. Selection Type: Strains were either under positive or neutral artificial selection for dispersal.
Download Cross Inoculation Data.csv (1.548 Kb)
Details View File Details
Title Vmax Data
Downloaded 30 times
Description Vmax Data.csv, Data description: Data used to create Figure 2: Average growth rate of evolved strains in vitro relative to the ancestral strain. The data gives the growth rate of the evolved strains relative to the ancestral strain from which all strains were derived. There are 6 replicate lines, which were averaged to give “Growth Rate Relative to Ancestor”. Data was collected in the laboratory using a spectrophotometer. Columns correspond to: Strain Replicate; the name allocated to each of the replicate lines, e.g. H1 corresponds to replicate line 1 evolved on hard agar. Vmax Replicate: The Vmax was measured independently twice in order to provide replicates. This value was averaged in statistical analysis. Agar Type on which Strain was Evolved: Strains were either evolved in hard or liquid agar. Vmax Relative to Ancestor: Vmax of evolved strain under each treatment group was divided by Vmax of ancestral strain under the same treatment to give a relative measurement of area dispersed. Averaged Vmax Replicates: Replicate relative Vmax measurements were averaged. Selection Type: Strains were either under positive or neutral artificial selection for dispersal.
Download Vmax Data.csv (1.492 Kb)
Details View File Details
Title Biofilm Data
Downloaded 27 times
Description Biofilm Data.csv, Data description: Data used to create Figure 3: Biofilm production of evolved strains. Results show the average biofilm production of 6 isolates evolved in each experimental environment, relative to the ancestral strain from which all evolved strains were derived. Data was collected in the laboratory using a crystal violet staining method. Columns correspond to: Strain Replicate: the name allocated to each of the replicate lines, e.g. H1 corresponds to replicate line 1 evolved on hard agar. Bioflim Replicate: The biofilm production was measured independently eight times in order to provide replicates. This value was averaged in statistical analysis. Agar Type on which Strain was Evolved: Strains were either evolved in hard or liquid agar. Biofilm Relative to Ancestor: Vmax of evolved strain under each treatment group was divided by Vmax of ancestral strain under the same treatment to give a relative measurement of area dispersed. Averaged Biofilm Replicates: Replicate relative biofilm measurements were averaged. Selection Type: Strains were either under positive or neutral artificial selection for dispersal.
Download Biofilm Data.csv (5.089 Kb)
Details View File Details
Title In vivo Growth Data
Downloaded 26 times
Description In vivo Growth Data.csv, Data description: Data used to create Figure 4: Average growth rate of evolved strains within a host (G. mellonella) relative to the ancestral strain. Data was collected in the laboratory. Columns correspond to: Strain Replicate: the name allocated to each of the replicate lines, e.g. H1 corresponds to replicate line 1 evolved on hard agar. In vivo Replicate: The in vivo growth rate production was measured independently four times in order to provide replicates. This value was averaged in statistical analysis. Agar Type on which Strain was Evolved: Strains were either evolved in hard or liquid agar. In vivo Relative to Ancestor: In vivo growth rate of evolved strain under each treatment group was divided by In vivo growth rate of ancestral strain under the same treatment to give a relative measurement of area dispersed. Averaged In vivo Replicates: Replicate relative in vivo growth rate measurements were averaged. Selection Type: Strains were either under positive or neutral artificial selection for dispersal. Caterpillar Fresh Weight: The weight of each caterpillar was measured before inoculation to control for the effects of caterpillar size on bacterial growth.
Download In vivo Growth Data.csv (6.329 Kb)
Details View File Details

When using this data, please cite the original publication:

Taylor TB and Buckling A (2011) Selection experiments reveal trade-offs between swimming and twitching motilities in Pseudomonas aeruginosa. Evolution 65(11): 3060–3069. http://dx.doi.org/10.1111/j.1558-5646.2011.01376.x

Additionally, please cite the Dryad data package:

Taylor TB, Buckling A (2011) Data from: Selection experiments reveal trade-offs between swimming and twitching motilities in Pseudomonas aeruginosa. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.230t0
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: