Data from: Lake sediment multi-taxon DNA from North Greenland records early post-glacial appearance of vascular plants and accurately tracks environmental changes

Epp LS, Gussarova G, Boessenkool S, Olsen J, Haile J, Schrøder-Nielsen A, Ludikova A, Hassel K, Stenøien HK, Funder SV, Willerslev E, Kjær K, Brochmann C

Date Published: May 6, 2015

DOI: http://dx.doi.org/10.5061/dryad.26h7b

 

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Title Bliss_bryo_arcticborealbryo.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers bryo_P6F_1* & bryo_P6R as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. Taxonomic identification of sequences was inferred using a compilation of quality-checked and curated reference libraries for arctic and boreal species constructed at the Natural History Museum in Oslo (arctic vascular plants: Sønstebø et al. 2010; boreal vascular plants: Willerslev et al. 2014; bryophytes: Soininen et al. 2015). Further details can be found in the ReadMe file.
Download Bliss_bryo_arcticborealbryo.tag.fasta (16.34 Kb)
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Title Bliss_bryo_embl113.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers bryo_P6F_1* & bryo_P6R as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. Taxonomic identification of sequences was inferred using a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Further details can be found in the ReadMe file.
Download Bliss_bryo_embl113.tag.fasta (15.59 Kb)
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Title Bliss_vp1_arcticborealbryo.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers trnL_g and trnL_h as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. These sequences were recovered in the first round of amplification and sequencing of vascular plant DNA, as detailed in the associated publication (vascular plant dataset 1, "vp1"). Taxonomic identification of sequences was inferred using a compilation of quality-checked and curated reference libraries for arctic and boreal species constructed at the Natural History Museum in Oslo (arctic vascular plants: Sønstebø et al. 2010; boreal vascular plants: Willerslev et al. 2014; bryophytes: Soininen et al. 2015). Further details can be found in the ReadMe file.
Download Bliss_vp1_arcticborealbryo.tag.fasta (94.58 Kb)
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Title Bliss_vp1_embl113.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers trnL_g and trnL_h preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. These sequences were recovered in the first round of amplification and sequencing of vascular plant DNA, as detailed in the associated publication (vascular plant dataset 1, "vp1"). Taxonomic identification of sequences was inferred using a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Further details can be found in the ReadMe file.
Download Bliss_vp1_embl113.tag.fasta (94.66 Kb)
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Title Bliss_vp2_arcticborealbryo.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers trnL_g and trnL_h as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. These sequences were recovered in the second round of amplification and sequencing of vascular plant DNA, as detailed in the associated publication (vascular plant dataset 2, "vp2"). Taxonomic identification of sequences was inferred using a compilation of quality-checked and curated reference libraries for arctic and boreal species constructed at the Natural History Museum in Oslo (arctic vascular plants: Sønstebø et al. 2010; boreal vascular plants: Willerslev et al. 2014; bryophytes: Soininen et al. 2015). Further details can be found in the ReadMe file.
Download Bliss_vp2_arcticborealbryo.tag.fasta (79.98 Kb)
Download README (3.905 Kb)
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Title Bliss_vp2_embl113.tag
Description The unique P6 loop trnL sequences produced by amplification of DNA with the primers trnL_g and trnL_h preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. These sequences were recovered in the second round of amplification and sequencing of vascular plant DNA, as detailed in the associated publication (vascular plant dataset 2, "vp2"). Taxonomic identification of sequences was inferred using a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Further details can be found in the ReadMe file.
Download Bliss_vp2_embl113.tag.fasta (78.74 Kb)
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Title Bliss_diat_embl113.tag
Description The unique rbcL sequences produced by amplification of DNA with the primers rbcL_705f & rbcl_808r as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. Taxonomic identification of sequences was inferred using a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Further details can be found in the ReadMe file.
Download Bliss_diat_embl113.tag.fasta (153.2 Kb)
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Title Bliss_cop_embl113.tag
Description The unique sequences produced by amplification of DNA with the primers CopF2 & CopR1 as detailed in the associated publication. The data was recovered from DNA preserved in a sediment core spanning the Holocene from Bliss Lake, Peary Land, North Greenland. Taxonomic identification of sequences was inferred using a reference library formatted from the EMBL Nucleotide Database standard release 113 by extracting sequences of the targeted region using ecoPCR (Ficetola et al. 2010). Further details can be found in the ReadMe file.
Download Bliss_cop_embl113.tag.fasta (8.083 Kb)
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When using this data, please cite the original publication:

Epp LS, Gussarova G, Boessenkool S, Olsen J, Haile J, Schrøder-Nielsen A, Ludikova A, Hassel K, Stenøien HK, Funder SV, Willerslev E, Kjær K, Brochmann C (2015) Lake sediment multi-taxon DNA from North Greenland records early post-glacial appearance of vascular plants and accurately tracks environmental changes. Quaternary Science Reviews 117: 152–163. http://dx.doi.org/10.1016/j.quascirev.2015.03.027

Additionally, please cite the Dryad data package:

Epp LS, Gussarova G, Boessenkool S, Olsen J, Haile J, Schrøder-Nielsen A, Ludikova A, Hassel K, Stenøien HK, Funder SV, Willerslev E, Kjær K, Brochmann C (2015) Data from: Lake sediment multi-taxon DNA from North Greenland records early post-glacial appearance of vascular plants and accurately tracks environmental changes. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.26h7b
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