Data from: Evolution and diversification of the organellar release factor family

 

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Title RF1 subfamily alignment (mtRF1a, mtRF1 and pRF1)
Downloaded 31 times
Description Fasta multiple sequence alignment of Release Factor 1 proteins. Sequences were retrieved from GenBank nr database using PSI-BLAST. The alignment was obtained with ClustalW (v2.0.10), and 60% of the gaps was removed with BMGE (v1.0). The alignment was visually inspected and manually adjusted to accuratly align all functionally characterized domains and sequence motifs. Fasta multiple sequence alignment of Release Factor 2 proteins. Sequences were retrieved from GenBank nr database using PSI-BLAST. The alignment was obtained with ClustalW (v2.0.10), and 60% of the gaps was removed with BMGE (v1.0). The alignment was visually inspected and manually adjusted to accuratly align all functionally characterized domains and sequence motifs. Each sequence header contains the species name, the identity of each protein and, for bacterial species, the bacterial group they belong to. The numbers present in the header are not meaningful.
Download mtrf1a_mtrf1_prf1.bmge60gapRemoved.aln.fasta (78.87 Kb)
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Title RF2 subfamily alignment (mtrf2a, mtrf2b, mtrf2c, prf2)
Downloaded 22 times
Description Fasta multiple sequence alignment of Release Factor 2 proteins. Sequences were retrieved from GenBank nr database using PSI-BLAST. The alignment was obtained with ClustalW (v2.0.10), and 60% of the gaps was removed with BMGE (v1.0). The alignment was visually inspected and manually adjusted to accuratly align all functionally characterized domains and sequence motifs. Each sequence header contains the species name, the identity of each protein and, for bacterial species, the bacterial group they belong to. The numbers present in the header are not meaningful.
Download mtrf2c_mtrf2a_prf2_mtrf2b.bmge60gapRemoved...fasta (42.70 Kb)
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Title Eukaryotic Complete Release Factor family alignment (RF1, RF2, ICT1, C12orf65)
Downloaded 19 times
Description Fasta multiple sequence alignment of all eukaryotic Release Factor proteins. Sequences were retrieved from GenBank nr database using PSI-BLAST. These were aligned through profile to profile sequential alignment of the individual subfamilies’ alignments using ClustalW (v2.0.10) followed by one last round of alignment refinement with Muscle’s refine option. Finally, the whole RF family alignment was inspected and manually adjusted. No gaps were removed. Each sequence header contains the species name and the identity of each protein.
Download rf1_rf2_ict1_c12orf65-EukOnly.full-seqs.aln.fasta (187.3 Kb)
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When using this data, please cite the original publication:

Duarte I, Nabuurs SB, Magno R, Huynen M (2012) Evolution and diversification of the organellar release factor family. Molecular Biology and Evolution 29(11): 3497-3512. http://dx.doi.org/10.1093/molbev/mss157

Additionally, please cite the Dryad data package:

Duarte I, Nabuurs SB, Magno R, Huynen M (2012) Data from: Evolution and diversification of the organellar release factor family. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.2br48
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