Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods

Piñol J, Mir G, Gomez-Polo P, Agusti N

Date Published: December 2, 2014

DOI: http://dx.doi.org/10.5061/dryad.2p51n

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title chip1_bp00x_t45_c40
Downloaded 6 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 00x annealing temperature = 45 PCR cycles = 40
Download chip1_bp00x_t45_c40.fastq (40.29 Mb)
Details View File Details
Title chip1_bp01x_t45_c40
Downloaded 6 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 01x annealing temperature = 45 PCR cycles = 40
Download chip1_bp01x_t45_c40.fastq (45.68 Mb)
Details View File Details
Title chip1_bp02x_t45_c40
Downloaded 4 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 02x annealing temperature = 45 PCR cycles = 40
Download chip1_bp02x_t45_c40.fastq (36.32 Mb)
Details View File Details
Title chip1_bp05x_t45_c40
Downloaded 4 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 05x annealing temperature = 45 PCR cycles = 40
Download chip1_bp05x_t45_c40.fastq (39.93 Mb)
Details View File Details
Title chip1_bp10x_t45_c40
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 10x annealing temperature = 45 PCR cycles = 40
Download chip1_bp10x_t45_c40.fastq (40.42 Mb)
Details View File Details
Title chip1_bp20x_t45_c40
Downloaded 4 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 20x annealing temperature = 45 PCR cycles = 40
Download chip1_bp20x_t45_c40.fastq (42.47 Mb)
Details View File Details
Title chip1_bp40x_t45_c40
Downloaded 4 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 1 blocking primer concentration = 40x annealing temperature = 45 PCR cycles = 40
Download chip1_bp40x_t45_c40.fastq (38.05 Mb)
Details View File Details
Title chip2_bp00x_t40_c40
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 40 PCR cycles = 40
Download chip2_bp00x_t40_c40.fastq (31.06 Mb)
Details View File Details
Title chip2_bp00x_t45_c25
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 45 PCR cycles = 25
Download chip2_bp00x_t45_c25.fastq (16.63 Mb)
Details View File Details
Title chip2_bp00x_t45_c30
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 45 PCR cycles = 30
Download chip2_bp00x_t45_c30.fastq (16.91 Mb)
Details View File Details
Title chip2_bp00x_t45_c35
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 45 PCR cycles = 35
Download chip2_bp00x_t45_c35.fastq (23.10 Mb)
Details View File Details
Title chip2_bp00x_t45_c40
Downloaded 6 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 45 PCR cycles = 40
Download chip2_bp00x_t45_c40.fastq (27.98 Mb)
Details View File Details
Title chip2_bp00x_t50_c40
Downloaded 3 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 50 PCR cycles = 40
Download chip2_bp00x_t50_c40.fastq (31.28 Mb)
Details View File Details
Title chip2_bp00x_t55_c40
Downloaded 5 times
Description Raw data in fastq format from the sequencing of the mock sample using a 314 Ion Torrent chip. PCR conditions (see table 1 for details): chip = 2 blocking primer concentration = 00x annealing temperature = 55 PCR cycles = 40
Download chip2_bp00x_t55_c40.fastq (29.59 Mb)
Details View File Details
Title Barcodes of the 11 species in the mock sample
Downloaded 9 times
Description Database with the COI barcodes of the eleven species obtained by Sanger sequencing (fasta file).
Download 11_species.fasta (7.932 Kb)
Details View File Details
Title Barcodes of 188 Entomobryidae from Genbank
Downloaded 7 times
Description Database with the COI barcodes of 188 species of Entomobryidae (Collembola) obtained from Genbank (fasta file). Search: Entomobryidae[Organism] AND COI[Gene Name] AND Barcode[Keyword] (accessed on July 30th, 2014)
Download entomobryidae_20140730_coi_barcode.fasta (152.1 Kb)
Details View File Details

When using this data, please cite the original publication:

Piñol J, Mir G, Gomez-Polo P, Agusti N (2015) Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods. Molecular Ecology Resources 15(4): 819-830. http://dx.doi.org/10.1111/1755-0998.12355

Additionally, please cite the Dryad data package:

Piñol J, Mir G, Gomez-Polo P, Agusti N (2014) Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.2p51n
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: