Data from: Determining the subcellular location of new proteins from microscope images using local features

Coelho LP, Kangas JD, Naik AW, Osuna-Highley E, Glory-Afshar E, Fuhrman M, Simha R, Berget PB, Jarvik JW, Murphy RF

Date Published: August 30, 2013

DOI: http://dx.doi.org/10.5061/dryad.2vm70

 

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Title Labeled RandTag Widefield Images (Unlabeled Subset)
Downloaded 58 times
Description This datasets consists of fluorescent microscope images of GFP-tagged proteins locating to different organelles. The organelles were annotated by visual inspection. For each organelle, multiple proteins locating to it were identified; and for each protein, multiple images were acquired. Images contain two channels: GFP tagged protein and a nuclear marker (Hoechst). NIH 3T3 cells were tagged using CD tagging. This data was acquired on a widefield microscope
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Title Labeled RandTag Confocal Images
Downloaded 36 times
Description This datasets consists of fluorescent microscope images of GFP-tagged proteins locating to different organelles. The organelles were annotated by visual inspection. For each organelle, multiple proteins locating to it were identified; and for each protein, multiple images were acquired. Images contain two channels: GFP tagged protein and a nuclear marker (Hoechst). NIH 3T3 cells were tagged using CD tagging. This data was acquired on a confocal microscope.
Download labeled-confocal.tar.bz2 (646.2 Mb)
Download README.rst (1.375 Kb)
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Title Labeled RandTag Widefield Images (Nuclear Subset)
Downloaded 26 times
Description Part of widefield labeled dataset
Download nuclear.tar.bz2 (256.2 Mb)
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Title Labeled RandTag Widefield Images (Cytoskeleton Subset)
Downloaded 36 times
Description This is part of the labeled widefield dataset.
Download cytoskeleton.tar.bz2 (211.2 Mb)
Download README (1.915 Kb)
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Title Labeled RandTag Widefield Images (Nucleoli Subset)
Downloaded 27 times
Description This is part of the labeled widefield dataset.
Download nucleoli.tar.bz2 (182.0 Mb)
Download README (1.915 Kb)
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Title Labeled RandTag Widefield Images (Golgi Subset)
Downloaded 26 times
Description This file is part of the labeled widefield dataset.
Download Golgi.tar.bz2 (180.4 Mb)
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Title Labeled RandTag Widefield Images (Mitochondria Subset)
Downloaded 25 times
Description Part of widefield labeled subset
Download mitochondria.tar.bz2 (178.1 Mb)
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Title Labeled RandTag Widefield Images (Cytoplasmic Subset)
Downloaded 17 times
Description This is part of the labeled widefield dataset.
Download cytoplasmic.tar.bz2 (155.3 Mb)
Download README (1.915 Kb)
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Title Labeled RandTag Widefield Images (Lysosomal Subset)
Downloaded 23 times
Description This is part of the labeled widefield dataset
Download lysosome.tar.bz2 (149.5 Mb)
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Title Labeled RandTag Widefield Images (Plasma Membrane Subset)
Downloaded 16 times
Description This file is part of the widefield labeled dataset
Download membrane.tar.bz2 (108.2 Mb)
Download README (1.915 Kb)
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Title Labeled RandTag Widefield Images (ER Subset)
Downloaded 19 times
Description This is part of the labeled widefield dataset.
Download ER.tar.bz2 (94.85 Mb)
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When using this data, please cite the original publication:

Coelho LP, Kangas JD, Naik AW, Osuna-Highley E, Glory-Afshar E, Fuhrman M, Simha R, Berget PB, Jarvik JW, Murphy RF (2013) Determining the subcellular location of new proteins from microscope images using local features. Bioinformatics 29(18): 2343-2349. http://dx.doi.org/10.1093/bioinformatics/btt392

Additionally, please cite the Dryad data package:

Coelho LP, Kangas JD, Naik AW, Osuna-Highley E, Glory-Afshar E, Fuhrman M, Simha R, Berget PB, Jarvik JW, Murphy RF (2013) Data from: Determining the subcellular location of new proteins from microscope images using local features. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.2vm70
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