Data from: Postcopulatory sexual selection generates speciation phenotypes in Drosophila

Manier MK, Lüpold S, Belote JM, Starmer WT, Berben KS, Ala-Honkola O, Collins WF, Pitnick S

Date Published: October 4, 2013

DOI: http://dx.doi.org/10.5061/dryad.32665

 

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Title Remate
Downloaded 20 times
Description Sperm numbers in space and time after second mating. Variables: Cross - Species identity of first and second male to mate with a D. simulans female, where "s" indicates D. simulans and "m" indicates D. mauritiana, such that "ss" indicates that both males were D. simulans, "ms" indicates that the first male was D. mauritiana and the second male was D. simulans, etc. MinASM – time after the start of mating at which female was frozen, in minutes. Remate – day after first mating (day 0) on which female remated. Cop1 – duration in minutes of the first copulation. Cop2 – duration in minutes of the second copulation. PriorProg – number of progeny produced by female before remating. Matings - number of successful matings by that female. ThoraxF - female thorax length, in arbitrary units. ThoraxM1 - thorax length of the first male, in arbitrary units. ThoraxM2 - thorax length of the second male, in arbitrary units. Bursa1 – number of first-male sperm in the bursa. Bursa2 – number of second-male sperm in the bursa. SR1 – number of first-male sperm in the SR. SR2 – number of second-male sperm in the SR. Sp1 – number of first-male sperm in the spermathecae. Sp2 – number of second-male sperm in the spermathecae
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Title Virgin
Downloaded 19 times
Description Data on sperm transfer and storage after single mating of D. simulans female with D. mauritiana male. Variables: Treat – treatment, storage or transfer. Cop – copulation duration in minutes. ThoraxF - female thorax length, in arbitrary units. ThoraxM - thorax length of the second male, in arbitrary units. Bursa – number of sperm in the bursa. SR – number of sperm in the seminal receptacle. Sp – number of sperm in the spermathecae
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Title Ejection1
Downloaded 12 times
Description Timing and number of sperm ejected after a single mating of a D. simulans female with a D. mauritiana male. Variables: CopDur - copulation duration in minutes. EjTime - minutes after start of copulation when female ejected sperm mass. Sperm – number of sperm in the ejected sperm mass
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Title Ejection2
Downloaded 10 times
Description Timing and numbers of first- and second-male sperm ejected after remating, and numbers of first- and second-male sperm remaining in the female reproductive tract after ejection. Variables: Cross – Species identity of first and second male to mate with a D. simulans female, where "s" indicates D. simulans and "m" indicates D. mauritiana, such that "ss" indicates that both males were D. simulans, "ms" indicates that the first male was D. mauritiana and the second male was D. simulans, etc. Remate – day after first mating (day 0) on which female remated. Eject - 1 if female ejected, 0 if she did not eject. EjStart - Minutes after start of mating when female began ejecting a sperm mass (sometimes it got stuck). EjEnd - Minutes after start of mating when female finished ejecting a sperm mass. PriorProg - presence of prior progeny, where 0 = none, 1 = a few, 2 = a lot. Eject1 – number of first-male sperm in ejected sperm mass. Eject2 - number of second-male sperm in ejected sperm mass. ThoraxF - female thorax length, in arbitrary units. ThoraxM - thorax length of the second male, in arbitrary units. Bursa1 – number of first-male sperm in the bursa. Bursa2 – number of second-male sperm in the bursa. SR1 – number of first-male sperm in the SR. SR2 – number of second-male sperm in the SR. Sp1 – number of first-male sperm in the spermathecae. Sp2 – number of second-male sperm in the spermathecae
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Title Velocity
Downloaded 10 times
Description Sperm velocity and sperm numbers in the SR. Variables: Treat - sxm indicates single mating between D. simulans female and D. mauritiana male, NR indicates "no remate" treatment, other treatments are rematings defined by the species identity of first and second male to mate with a D. simulans female, where "s" indicates D. simulans and "m" indicates D. mauritiana, such that "ss" indicates that both males were D. simulans, "ms" indicates that the first male was D. mauritiana and the second male was D. simulans, etc. Region – region of the female reproductive tract, bursa or SR. Male – species and color tag of male whose sperm velocity is recorded. Order - first or second male to mate. Remate - for remating treatments, day after first mating (day 0) on which female remated. Velmps – instantaneous linear velocity in microns per second, averaged for each sperm and for all sperm recorded in a movie. N – number of sperm tracked per movie. SR.G – number of GFP sperm in the SR. SR.R – number of RFP sperm in the SR.
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Title mxs
Downloaded 11 times
Description Numbers of sperm transferred and stored for D. mauritiana females mating with D. simulans males. Variables: Cross - mxs = D. mauritiana female mating once with a D. simulans male; mxsm = D. mauritiana female mating first with a D. simulans male followed by a D. mauritiana male; mxms = D. mauritiana female mating first with a D. mauritiana male followed by a D. simulans male. Cop - copulation duration in minutes, for second copulation for mxsm and mxms crosses. Bursa1 – number of first-male sperm in the bursa. Bursa2 – number of second-male sperm in the bursa. SR1 – number of first-male sperm in the SR. SR2 – number of second-male sperm in the SR. Sp1 – number of first-male sperm in the spermathecae. Sp2 – number of second-male sperm in the spermathecae
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Title P2
Downloaded 14 times
Description Paternity and sperm count data. Variables: Cross - Species identity of first and second male to mate with a D. simulans female, where "s" indicates D. simulans and "m" indicates D. mauritiana, such that "ss" indicates that both males were D. simulans, "ms" indicates that the first male was D. mauritiana and the second male was D. simulans, etc. Treat – time after mating for which paternity and sperm numbers in the female reproductive tract were quantified. ThoraxF - female thorax length, in arbitrary units. ThoraxM1 - thorax length of the first male, in arbitrary units. ThoraxM2 - thorax length of the second male, in arbitrary units. Cop1 – duration in minutes of the first copulation. Cop2 – duration in minutes of the second copulation. Remate – day after first mating (day 0) on which female remated. PriorProg – number of progeny produced by female before remating. P2Prog1 - number of progeny produced after remating sired by the first male. P2Prog2 - number of progeny produced after remating sired by the second male. P2 – proportion of progeny produced after remating sired by the second male. Bursa1 – number of first-male sperm in the bursa. Bursa2 – number of second-male sperm in the bursa. SR1 – number of first-male sperm in the SR. SR2 – number of second-male sperm in the SR. Sp1 – number of first-male sperm in the spermathecae. Sp2 – number of second-male sperm in the spermathecae.
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When using this data, please cite the original publication:

Manier MK, Lüpold S, Belote JM, Starmer WT, Berben KS, Ala-Honkola O, Collins WF, Pitnick S (2013) Postcopulatory sexual selection generates speciation phenotypes in Drosophila. Current Biology 23(19): 1853–1862. http://dx.doi.org/10.1016/j.cub.2013.07.086

Additionally, please cite the Dryad data package:

Manier MK, Lüpold S, Belote JM, Starmer WT, Berben KS, Ala-Honkola O, Collins WF, Pitnick S (2013) Data from: Postcopulatory sexual selection generates speciation phenotypes in Drosophila. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.32665
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