Data from: Low-coverage, whole-genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery

Gardner EM, Johnson MG, Ragone D, Wickett NJ, Zerega NJC

Date Published: July 13, 2016

DOI: http://dx.doi.org/10.5061/dryad.3293r

 

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Title Genome assemblies, annotations, targets, and bait sequences from Gardner et al. 2016
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Description A_camansi_v1.0_scaffolds.fasta -- Genome assembly v. 1.0 (Ray 2.3.1) A_camansi_v1.1_scaffolds.fasta -- Genome assembly v. 1.1 (L_RNA_Scaffolder) A_camansi_v1.1_genepredictions.gff -- Gene predictions in GFF format (Augustus) A_camansi_v1.1_genepredictions.fna -- Predicted coding sequences (Augustus) A_camansi_v1.1_genepredictions.faa.aa -- Predicted protein sequences (Augustus) A_camansi_v1.1_genepredictions_annotations.txt -- Gene annotations (Trinotate) artocarpus_333genes.fasta -- 333 phylogenetic marker sequences artocarpus_mbgenes.fasta -- 98 putativeMADS-box gene sequences artocarpus_volgenes.fasta -- 27 putative volatile gene sequences artocarpus_baits.fasta -- 120mer bait sequences (designed by MYcroarray)
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Download Gardner_et_al_2016.tar.gz (427.6 Mb)
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Title HybPiper assemblies for Johnson et al. (2016)
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Description Output of HybPiper for 22 Artocarpus species and six outgroups, including Ficus and Morus. BWA was used to map the reads to the targets, including 333 genes used for phylogenetics (prefix "gene"), 98 MADS-Box genes (prefix "MB") and 27 genes functionally annotated as involved in volatile compounds (prefix "Vol"). After running the main HybPiper script (reads_first.py) we also ran "intronerate.py" to extract exon sequences, "paralog_investigator.py" to extract putative paralog sequences, "depth_calculator.py" to estimate depth of coverage in recovered exon sequence, and "cleanup.py" to remove redundant files from the SPAdes contig assembly. Data from: Johnson M., E.M. Gardner, J. Shaw, Y. Liu, R. Medina, B. Goffinet, N.J.C. Zerega, and N. Wickett. HybPiper: extracting phylogenetic datasets from high-throughput sequencing reads using targeted bait capture. Applications in Plant Sciences
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Title Analyses from Johnson et al. 2016
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Description README for HybPiper_artocarpus_analysis.tar.gz artocarpus_trimmed.exon.tar.gz Trimmed coding sequence (exon) alignments for 28 taxa for 333 genes. Sequences were aligned in MAFFT and trimmed using Trimal, discarding all columns with more than 80% missing data. supercontig_trimmed_fasta.tar.gz Trimmed supercontig sequence alignments (containing both exons and flanking "splash zone" intron sequence) for 28 taxa for 333 genes. Sequences were aligned in MAFFT and trimmed using Trimal, discarding all columns with more than 80% missing data. artocarpus_hybpiper_genelengths.txt Lengths of exon sequence (CDS) recovered for 458 loci for 28 taxa using the BWA method. File generated using "get_seq_lengths.py" in HybPiper, and used to generate the heatmap figure in the HybPiper manuscript (with "gene_recovery_heatmap.R") allbaitsuppercase.fna Nucleotide "target" file used with HybPiper. For 333 loci, there are two orthologous sequences per gene: one from the Artocarpus camansi draft genome, and one from the Morus notabalis genome. For the remaining genes, only an Artocarpus ortholog is present. artocarpus_bwa.supercontig.supermatrix.raxml.tre RAxML phylogeny generated from a concatenated supermatrix of supercontig sequences for 22 Artocarpus species and six outgroups, using the 333 "phylogenetic" loci. Tree generated from nucleotide data using the GTRCAT model, with one partition per gene. artocarpus_hybseq.exon.raxml.names.tre RAxML phylogeny generated from a concatenated supermatrix of exon sequences extracted by HybPiper for 22 Artocarpus species and six outgroups, using the 333 "phylogenetic" loci. Node labels indicate bootstrap support from 200 "fast bootstrap" replicates. Analyses from: Johnson M., E.M. Gardner, J. Shaw, Y. Liu, R. Medina, B. Goffinet, N.J.C. Zerega, and N. Wickett. HybPiper: extracting phylogenetic datasets from high-throughput sequencing reads using targeted bait capture. Applications in Plant Sciences
Download HybPiper_artocarpus_analysis.tar.gz (3.497 Mb)
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When using this data, please cite the original publication:

Gardner EM, Johnson MG, Ragone D, Wickett NJ, Zerega NJC (2016) Low-coverage, whole-genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery. Applications in Plant Sciences 4(7): 1600017. http://dx.doi.org/10.3732/apps.1600017

Additionally, please cite the Dryad data package:

Gardner EM, Johnson MG, Ragone D, Wickett NJ, Zerega NJC (2016) Data from: Low-coverage, whole-genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.3293r
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