Data from: What you need is what you eat? Prey selection by the bat Myotis daubentonii

Vesterinen EJ, Ruokolainen L, Wahlberg N, Peña C, Roslin T, Laine VN, Vasko V, Sääksjärvi IE, Norrdahl K, Lilley TM

Date Published: February 2, 2016

DOI: http://dx.doi.org/10.5061/dryad.343nd

 

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Title Raw labeled reads from Insect trap samples
Downloaded 21 times
Description Collected on daily basis using three emergence traps and one Malaise trap. DNA extracted from bulk samples using a modified salt-extraction method. Sequenced using Ion Torrent PGM. FASTQ file header stands for: @READNUMBER;barcodelabel=TRAPTYPE-DAY_MONTH.
Download labeled_insect.fastq (1.817 Gb)
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Title Raw labeled reads from faecal samples of Myotis daubentonii
Downloaded 8 times
Description Collected on daily basis one single location. DNA extracted from individual pellets using Qiagen Stool Mini Kit. Sequenced using Ion Torrent PGM. FASTQ file header stands for: @READNUMBER;barcodelabel=SAMPLEID-DAY_MONTH.
Download labeled_poop.fastq (1.786 Gb)
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Title OTU sequences
Downloaded 7 times
Description Clustered to OTUs using USEARCH algorithm with default (97%) similarity. This way there is a little bit of oversplitting, and OTUs belonging to same biological species are clustered subsequently.
Download label_otus.fa (102.2 Kb)
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Title Readmap linking trimmed reads and samples
Downloaded 14 times
Description The readmap shows read count for each sample. The readmap is constructed using USEARCH software.
Download readmap.tab (187.7 Kb)
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Title Technical data sheet for IonTorrent PGM runs
Downloaded 111 times
Description The data generated by Ion Torrent PGM for each run in hte study.
Download Ion_Torrent_runs.pdf (1.195 Mb)
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Title Command pipeline used for bioinformatics
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Description The file contains all the commandds used in this study to generate the final data. The bioinformatics was carried out at servers on CSC - IT Center for SCience in Finland <www.csc.fi>.
Download commands_unix_csc.sh (4.02 Kb)
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Title The final readmap
Downloaded 20 times
Description This readmap contains the final read counts for each sample. The difference to raw readmap is that some OTU's in this version have been clumped together.
Download readmap_spp.xlsx (158.7 Kb)
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Title Microsatellite Data for Myotis daubentonii faecal pellets
Downloaded 21 times
Description The zipped folder includes original consensus genotypes (cons_2Feb2015.txt), R commands to cluster individual bat haplotypes (Nautela_genotyping.R), and final genotypes (NautelaUniqueProfile_cons_2Feb2015_mismatch4.html).
Download NautelaMicrosatelliteData.zip (34.53 Kb)
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When using this data, please cite the original publication:

Vesterinen EJ, Ruokolainen L, Wahlberg N, Peña C, Roslin T, Laine VN, Vasko V, Sääksjärvi IE, Norrdahl K, Lilley TM (2016) What you need is what you eat? Prey selection by the bat Myotis daubentonii. Molecular Ecology 25(7): 1581–1594. http://dx.doi.org/10.1111/mec.13564

Additionally, please cite the Dryad data package:

Vesterinen EJ, Ruokolainen L, Wahlberg N, Peña C, Roslin T, Laine VN, Vasko V, Sääksjärvi IE, Norrdahl K, Lilley TM (2016) Data from: What you need is what you eat? Prey selection by the bat Myotis daubentonii. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.343nd
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