Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms

 

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Title PCR1
Downloaded 18 times
Description PCR1 mix: Bulk sample constituted from 30 diatoms strains corresponding to 12 genera and 21 species. 30 PCR products were mixed in varying molar proportions ranging from 0.1% to 8.8%. Then product of 3 markers (SSU rDNA, rbcL and cox1) were pooled in equimolar concentration. Fragmented PCR products were then sequenced in one random direction on a Roche GS FLX with Titanium chemistry on a GS FLX Titanium PicoTiterPlate 454 (Roche) at the Genomic Platform of Genopole Toulouse/Midi-Pyrenees
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Title PCR2
Downloaded 29 times
Download PCR2.fasta (111.7 Mb)
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Title CMix
Downloaded 20 times
Download CMix.fasta (49.68 Mb)
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When using this data, please cite the original publication:

Kermarrec L, Franc A, Rimet F, Chaumeil P, Humbert J, Bouchez A (2013) Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms. Molecular Ecology Resources 13(4): 607–619. http://dx.doi.org/10.1111/1755-0998.12105

Additionally, please cite the Dryad data package:

Kermarrec L, Franc A, Rimet F, Chaumeil P, Humbert J, Bouchez A (2013) Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.370nq
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