Data from: Wolbachia infection alters the relative abundance of resident bacteria in adult Aedes aegypti mosquitoes, but not larvae

Audsley MD, Seleznev A, Joubert DA, Woolfit M, O'Neill SL, McGraw EA

Date Published: November 22, 2017

DOI: https://doi.org/10.5061/dryad.37m5c

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Title larvae_unsum_fulldata_rawcounts
Downloaded 1 time
Description Larvae: Raw sequence counts assigned to OTUs (97% identity threshold) without any filtering or summarizing. Post Wolbachia sequence removal.
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Title adults_unsum_fulldata_rawcounts
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Description Adults: Raw sequence counts assigned to OTUs (97% identity threshold) without any filtering or summarizing. Post Wolbachia sequence removal.
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Title larvae_otu_table_noWol_L2
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Description Larvae: QIIME OTU table summarized at L2 (phylum). Post Wolbachia removal. No filtering for low count OTUs.
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Title larvae_otu_table_noWol_L3
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Description Larvae: QIIME OTU table summarized at L3 (class). Post Wolbachia removal. No filtering for low count OTUs.
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Title larvae_otu_table_noWol_L4
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Description Larvae: QIIME OTU table summarized at L4 (order). Post Wolbachia removal. No filtering for low count OTUs.
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Title larvae_otu_table_noWol_L5
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Description Larvae: QIIME OTU table summarized at L5 (family). Post Wolbachia removal. No filtering for low count OTUs.
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Title larvae_otu_table_noWol_L6
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Description Larvae: QIIME OTU table summarized at L6 (genus). Post Wolbachia removal. No filtering for low count OTUs.
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Title adults_otu_table_noWol_L2
Description Adults: QIIME OTU table summarized at L2 (phylum). Post Wolbachia removal. No filtering for low count OTUs.
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Title adults_otu_table_noWol_L3
Downloaded 1 time
Description Adults: QIIME OTU table summarized at L3 (class). Post Wolbachia removal. No filtering for low count OTUs.
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Title adults_dotu_table_noWol_L4
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Description Adults: QIIME OTU table summarized at L4 (order). Post Wolbachia removal. No filtering for low count OTUs.
Download otu_table_noWol_L4.txt (22.64 Kb)
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Title adults_otu_table_noWol_L5
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Description Adults: QIIME OTU table summarized at L5 (family). Post Wolbachia removal. No filtering for low count OTUs.
Download otu_table_noWol_L5.txt (62.71 Kb)
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Title adults_otu_table_noWol_L6
Downloaded 2 times
Description Adults: QIIME OTU table summarized at L6 (genus). Post Wolbachia removal. No filtering for low count OTUs.
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Title Larvae_unsum_edgeR_pooled_all
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Description Differential relative abundance calculated using phyloseq/edgeR with collection sites pooled to compare all Wolbachia-negative samples to all Wolbachia-positive samples. Input files were QIIME OTU tables (unsummarized, 97% identity threshold) that have been filtered for Wolbachia and for low-count OTUs.
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Title Larvae_unsum_edgeR_glm_all
Downloaded 3 times
Description Differential relative abundance in Wolbachia-negative samples compared to Wolbachia-positive samples, calculated using phyloseq/edgeR with a linear model to include collection site as a factor. Input files were QIIME OTU tables (unsummarized, 97% identity threshold) that have been filtered for Wolbachia and for low-count OTUs.
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Title Larvae_L6sum_edgeR_pooled_all
Downloaded 1 time
Description Differential relative abundance calculated using phyloseq/edgeR with collection sites pooled to compare all Wolbachia-negative samples to all Wolbachia-positive samples. Input files were QIIME OTU tables (summarized at L6, genus) that have been filtered for Wolbachia and for low-count OTUs.
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Title Larvae_L6sum_edgeR_glm_all
Downloaded 1 time
Description Differential relative abundance in Wolbachia-negative samples compared to Wolbachia-positive samples, calculated using phyloseq/edgeR with a linear model to include collection site as a factor. Input files were QIIME OTU tables (summarized at L6, genus) that have been filtered for Wolbachia and for low-count OTUs.
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Title Adults_unsum_edgeR_pooled_all
Downloaded 1 time
Description Differential relative abundance calculated using phyloseq/edgeR with collection sites pooled to compare all Wolbachia-negative samples to all Wolbachia-positive samples. Input files were QIIME OTU tables (unsummarized, 97% identity threshold) that have been filtered for Wolbachia and for low-count OTUs.
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Title Adults_unsum_edgeR_glm_all
Downloaded 1 time
Description Differential relative abundance in Wolbachia-negative samples compared to Wolbachia-positive samples, calculated using phyloseq/edgeR with a linear model to include collection site as a factor. Input files were QIIME OTU tables (unsummarized, 97% identity threshold) that have been filtered for Wolbachia and for low-count OTUs.
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Title Adults_L6sum_edgeR_pooled_all
Downloaded 1 time
Description Differential relative abundance calculated using phyloseq/edgeR with collection sites pooled to compare all Wolbachia-negative samples to all Wolbachia-positive samples. Input files were QIIME OTU tables (summarized at L6, genus) that have been filtered for Wolbachia and for low-count OTUs.
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Title Adults_L6sum_edgeR_glm_all
Downloaded 1 time
Description Differential relative abundance in Wolbachia-negative samples compared to Wolbachia-positive samples, calculated using phyloseq/edgeR with a linear model to include collection site as a factor. Input files were QIIME OTU tables (summarized at L6, genus) that have been filtered for Wolbachia and for low-count OTUs.
Download Adults_L6sum_edgeR_glm_all.xls (74.87 Kb)
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Title README
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Description README file pertaining to all uploads.
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When using this data, please cite the original publication:

Audsley MD, Seleznev A, Joubert DA, Woolfit M, O'Neill SL, McGraw EA (2017) Wolbachia infection alters the relative abundance of resident bacteria in adult Aedes aegypti mosquitoes, but not larvae. Molecular Ecology, online in advance of print. https://doi.org/10.1111/mec.14436

Additionally, please cite the Dryad data package:

Audsley MD, Seleznev A, Joubert DA, Woolfit M, O'Neill SL, McGraw EA (2017) Data from: Wolbachia infection alters the relative abundance of resident bacteria in adult Aedes aegypti mosquitoes, but not larvae. Dryad Digital Repository. https://doi.org/10.5061/dryad.37m5c
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