Data from: Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities

Lim NKM, Tay YC, Srivathsan A, Tan JWT, Kwik JTB, Baloğlu B, Meier R, Yeo DCJ

Date Published: November 3, 2016

DOI: http://dx.doi.org/10.5061/dryad.40hs5

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Title Bedok ngsfilter file
Downloaded 10 times
Description Demultiplexing reference file for Bedok Reservoir dataset, containing primer tag assignments. For use with the ngsfilter step of the obitools pipeline.
Download SI_Bedok_obitools-ngsfilter.txt (21.12 Kb)
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Title Pandan ngsfilter file
Downloaded 7 times
Description Demultiplexing reference file for Pandan Reservoir dataset, containing primer tag assignments. For use with the ngsfilter step of the obitools pipeline.
Download SI_Pandan_obitools-ngsfilter.txt (21.12 Kb)
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Title Aligned sequence set used for objective clustering
Downloaded 9 times
Description Final aligned COI sequence set comprising the combined Bedok and Pandan datasets, used for MOTU delimitation via objective clustering.
Download BKPN_metazoa_pullout_align.fa (4.475 Mb)
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Title Bedok obitools output
Downloaded 17 times
Description Cleaned Bedok dataset after obitools filtering.
Download BK_water_amp_ali_assigned_uniq_10_300_clean.fa (202.4 Mb)
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Title Pandan obitools output
Downloaded 18 times
Description Cleaned Pandan dataset after obitools filtering.
Download PN_water_amp_ali_assigned_uniq_10_300_clean.fa (180.5 Mb)
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When using this data, please cite the original publication:

Lim NKM, Tay YC, Srivathsan A, Tan JWT, Kwik JTB, Baloğlu B, Meier R, Yeo DCJ (2016) Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities. Royal Society Open Science 3(11): 160635. http://dx.doi.org/10.1098/rsos.160635

Additionally, please cite the Dryad data package:

Lim NKM, Tay YC, Srivathsan A, Tan JWT, Kwik JTB, Baloğlu B, Meier R, Yeo DCJ (2016) Data from: Next-generation freshwater bioassessment: eDNA metabarcoding with a conserved metazoan primer reveals species-rich and reservoir-specific communities. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.40hs5
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