Data from: TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst

Hermann C, van Hateren A, Trautwein N, Neerincx A, Duriez PJ, Stevanović S, Trowsdale J, Deane JE, Elliott T, Boyle LH

Date Published: October 8, 2015

DOI: http://dx.doi.org/10.5061/dryad.487j9

 

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Title Figure 5C - Peptides eluted from MHC class I molecules in Hela+IFNy and Hela-TAPBPR KO+IFNy
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Description Peptide-MHC class I complexes were isolated by affinity chromatography using W6/32 from IFN-γ treated HeLa and HeLa-TAPBPR KO cells. Eluted peptides were analysed using LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide motifs using the online programme NetMHC.
Download Hela IFNy and Hela IFNy TAPBPR KO.xlsx (115.8 Kb)
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Title Figure 5D - peptides eluted from HeLa and HeLa-WT-TAPBPR cells
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Description Peptide-MHC class I complexes were isolated by affinity chromatography using W6/32 from HeLa and HeLa overexpressing WT-TAPBPR. Eluted peptides were analysed using LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide motifs using the online programme NetMHC.
Download HeLa and HeLa WT TAPBPR.xlsx (47.81 Kb)
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Title Figure 5 - Figure supplement 1 - Peptides eluted from HeLa-S+IFNy and HeLa-S-shTAPBPR+IFNy
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Description Peptide-MHC class I complexes were isolated by affinity chromatography using W6/32 from IFN-γ treated HeLa-S cells and IFN-γ treated HeLa-S cells depleted of TAPBPR using shRNA. Eluted peptides were analysed using LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide motifs using the online programme NetMHC.
Download HeLa IFNy and HeLa INFy shTAPBPR.xlsx (100.9 Kb)
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Title Figure 6B - Peptide eluted from HLA-A2 from WT, TAPBPR depleted, and tapasin deficient KBM-7 cells
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Description Peptide loaded HLA-A2 complexes were isolated using affinity chromatography with BB7.2 from IFN-γ treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient (TPN-) KBM-7 cells. Eluted peptide were analysed using LC-MS/MS.
Download KBM7 elution from BB72.xlsx (30.63 Kb)
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Title Figure 6E - Peptides eluted from HLA-B & -C from WT, TAPBPR depleted and tapasin deficient KBM-7 cells
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Description HLA-B and -C complexes were isolated using affinity chromatography using B1.23.2 from IFN-γ treated WT, TAPBPR stably depleted (shTAPBPR) and tapasin deficient (TPN-) KBM-7 cells. Eluted peptide were analysed using LC-MS/MS.
Download KBM7 elution from B1232.xlsx (33.09 Kb)
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When using this data, please cite the original publication:

Hermann C, van Hateren A, Trautwein N, Neerincx A, Duriez PJ, Stevanović S, Trowsdale J, Deane JE, Elliott T, Boyle LH (2015) TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. eLife 4: e09617. http://dx.doi.org/10.7554/eLife.09617

Additionally, please cite the Dryad data package:

Hermann C, van Hateren A, Trautwein N, Neerincx A, Duriez PJ, Stevanović S, Trowsdale J, Deane JE, Elliott T, Boyle LH (2015) Data from: TAPBPR alters MHC class I peptide presentation by functioning as a peptide exchange catalyst. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.487j9
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