The study was conducted in an Atlantic Rainforest area, in the Serra do Mar State Park (23°20’S and 44°50’W), southeast of Brazil (São Paulo State coast), at altitudes between 43 and 89m asl (Eisenlohr et al. 2013). A trapping session of four consecutive nights was conducted monthly from October 2013 to November 2014. In order to capture the animals, we used one hundred Tomahawk traps (45 x 16 x 16 cm) on the ground, baited with banana, peanut butter, and bacon. The traps were 20 m apart from each other, forming a 3.4 ha grid (280m x 120m). Individuals captured were marked in each ear with one 1g monel ear tag (National Band and Tag Co.®), weighted, and identified by sex and age (Tyndale-Biscoe and MacKenzie 1976). Samples of scats were collected from adults only. Scats were collected from the bottom of the trap where the animal spent the night. The traps were placed on a platform of hardboard in order to isolate the scats from litter. Feces samples were dissolved in water and then filtered through a 1mm mesh screen sieve under running tap water. Then the material retained on the sieve was dried at ambient temperature. This undigested material was analyzed with a stereoscope and the taxonomic classification of the prey items found was done by specialists. After one year of trapping sessions (48 nights of trapping, 4800 trap-nights) we recorded 35 individuals (i.e., adults and juveniles) of *Didelphis aurita* and 19 individuals of *Metachirus nudicaudatus*. In order to avoid differences in the diet samplings of individuals, only adult feces were taken. Thirty-eight fecal samples were collected from 18 adults of *D. aurita*, and 32 fecal samples were collected from 15 adults of *M. nudicaudatus*. Their diet items are described in Table 01.