Data from: Comparative linkage maps suggest that fission, not polyploidy, underlies near-doubling of chromosome number within monkeyflowers (Mimulus; Phrymaceae)

Fishman L, Willis JH, Wu CA, Lee Y

Date Published: November 19, 2013

DOI: http://dx.doi.org/10.5061/dryad.4bh26

 

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Title Data for Composite Iron Mountain (CIM) M. guttatus linkage map
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Description Excel files with the individual x marker genotype matrices for three F2 mapping populations. See readme file for more info on the mapping populations and linkage map generation.
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Download CIM matrices.zip (640.1 Kb)
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Title Individual x genotype matrices for BG4 and BN4 nearly isogenic line (NIL) populations
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Description Both NIL populations were derived from the inbred line cross of Sherar's Falls (SF) Mimulus nasutus and Iron Mountain 62 (IM62) M. guttatus. The BG4 and BN4 lines started with IM62 x SF and SF x IM62 F1 hybrids, respectively, underwent 4 generations of backcrossing with the recurrent parent as pollen donor, and were made with single seed descent and no intentional selection. In both files, b = IM62 homozygote, a = SF homozygote, and H = heterozygote.
Download NIL matrices.zip (200.4 Kb)
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Title Individual x genotype matrix for NIM2 F2 mapping population (SF M. nasutus x IM62 M. guttatus)
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Description Matrix of genotypes for second SF x IM62 F2 mapping population. b = IM62 homozygote, a = M. nasutus homozygote, h = heterozygote. Some markers with extreme transmission ratio distortion relative to flanking markers are included in dataset, but were removed during mapping. Plant growth conditions Seeds were planted in 2.25-in pots filled with Fafard 4P potting mix, watered, and stratified in the dark at 4°C for one week. Pots were then moved to the Duke University greenhouses and maintained under long-day photoperiod conditions (16 h light at 24°C / 8 h dark at 16°C) using supplemental high-intensity sodium lights (daytime PPFD >750 mmol m-1 s-1). Light response curves indicate that this light level was near saturating for the plants (data not shown). We sowed approximately five seeds per pot, and thinned to the centermost individual after most seeds had germinated, two weeks after planting. While in the greenhouses, plants received weekly treatment with Peter’s Professional Blossom Booster 10-30-20 fertilizer.
Download NIM2_JoinMap.xls (1.762 Mb)
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When using this data, please cite the original publication:

Fishman L, Willis JH, Wu CA, Lee Y (2014) Comparative linkage maps suggest that fission, not polyploidy, underlies near-doubling of chromosome number within monkeyflowers (Mimulus; Phrymaceae). Heredity 112: 562–568. http://dx.doi.org/10.1038/hdy.2013.143

Additionally, please cite the Dryad data package:

Fishman L, Willis JH, Wu CA, Lee Y (2014) Data from: Comparative linkage maps suggest that fission, not polyploidy, underlies near-doubling of chromosome number within monkeyflowers (Mimulus; Phrymaceae). Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.4bh26
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