Daily rhythms in mammals are controlled by the circadian system, which is a collection of biological clocks regulated by a central pacemaker within the suprachiasmatic nucleus (SCN) of the anterior hypothalamus. Changes in SCN function have pronounced consequences for behaviour and physiology; however, few studies have examined whether individual differences in circadian behaviour reflect changes in SCN function. Here, PERIOD2::LUCIFERASE mice were exposed to a behavioural assay to characterize individual differences in baseline entrainment, rate of re-entrainment and free-running rhythms. SCN slices were then collected for ex vivo bioluminescence imaging to gain insight into how the properties of the SCN clock influence individual differences in behavioural rhythms. First, individual differences in the timing of locomotor activity rhythms were positively correlated with the timing of SCN rhythms. Second, slower adjustment during simulated jetlag was associated with a larger degree of phase heterogeneity among SCN neurons. Collectively, these findings highlight the role of the SCN network in determining individual differences in circadian behaviour. Furthermore, these results reveal novel ways that the network organization of the SCN influences plasticity at the behavioural level, and lend insight into potential interventions designed to modulate the rate of resynchronization during transmeridian travel and shift work..
PER2::LUC Imaging Mouse#337
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#337 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#337.zip
PER2::LUC Imaging Mouse#338
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#338 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#338.zip
PER2::LUC Imaging Mouse#340
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#340 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#340.zip
PER2::LUC Imaging Mouse#341
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#341 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#341.zip
PER2::LUC Imaging Mouse#342
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#342 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#342.zip
PER2::LUC Imaging Mouse#344
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#344 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#344.zip
PER2::LUC Imaging Mouse#345
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#345 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#345.zip
PER2::LUC Imaging Mouse#346
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#346 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#346.zip
PER2::LUC Imaging Mouse#349
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#349 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#349.zip
PER2::LUC Imaging Mouse#353
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#353 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#353.zip
PER2::LUC Imaging Mouse#360
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#360 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#360.zip
PER2::LUC Imaging Mouse#362
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#362 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#362.zip
PER2::LUC Imaging Mouse#363
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#363 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#363.zip
PER2::LUC Imaging Mouse#372
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#372 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#372.zip
PER2::LUC Imaging Mouse#373
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#373 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#373.zip
PER2::LUC Imaging Mouse#374
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#374 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#374.zip
PER2::LUC Imaging Mouse#375
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#375 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#375.zip
PER2::LUC Imaging Mouse#376
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#376 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#376.zip
PER2::LUC Imaging Mouse#377
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#377 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#377.zip
PER2::LUC Imaging Mouse#379
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#379 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#379.zip
PER2::LUC Imaging Mouse#380
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#380 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#380.zip
PER2::LUC Imaging Mouse#383
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#383 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#383.zip
PER2::LUC Imaging Mouse#384
This file contains a tiff sequence for PER2::LUC imagign data from SCN slices collected from Mouse#384 (rostral SCN = Slice1, middle SCN = Slice2, caudal SCN = Slice3).
#384.zip
Behavioral Data - Actograms
This file contains activity records for the in vivo phenotyping phase of this study. To view, it is best to open them with Clocklab software.