Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife

Razzauti M, Galan M, Bernard M, Maman S, Klopp C, Charbonnel N, Vayssier-Taussat M, Eloit M, Cosson J

Date Published: August 19, 2015

DOI: http://dx.doi.org/10.5061/dryad.50125

 

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Title 454 raw sequences of the V4 region 16S rRNA gene from the spleens of 190 bank voles
Downloaded 13 times
Description This FASTA file contains 78,087 raw sequences produced using 454 GS-FLX pyrosequencing. The 190 multiplexed amplicons were tagged using both forward and reverse primers. The list of the 190 multiplexed samples and associated tags are provided in the following CSV file titled: Information concerning the samples multiplexed in the 454 run.
Download 454_Reads_16Sv4_Myodes.fasta (26.59 Mb)
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Title Information concerning the samples multiplexed in the 454 run
Downloaded 9 times
Description This CSV file contains the sample names, the forward and reverse tag sequences, the forward and reverse primer sequences, the gene name, the species name and the population name for each of the 190 samples multiplexed in the 454 pyrosequencing run.
Download 454_Reads_16Sv4_Myodes.csv (19.54 Kb)
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Title MiSeq raw sequences of the V4 region 16S rRNA gene from the spleens of 190 bank voles (part 1)
Downloaded 61 times
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each individual using the MiSeq platform. The 190 multiplexed amplicons were indexed using both forward and reverse indexes. The list of the 190 multiplexed samples and associated indexes are provided in the following CSV file titled: Information concerning the samples multiplexed in the MiSeq run.
Download MiSeq_Reads_16Sv4_Myodes_part1.zip (997.1 Mb)
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Title MiSeq raw sequences of the V4 region 16S rRNA gene from the spleens of 190 bank voles (part 2)
Downloaded 45 times
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each individual using the MiSeq platform. The 190 multiplexed amplicons were indexed using both forward and reverse indexes. The list of the 190 multiplexed samples and associated indexes are provided in the following CSV file titled: Information concerning the samples multiplexed in the MiSeq run.
Download MiSeq_Reads_16Sv4_Myodes_part2.zip (982.1 Mb)
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Title MiSeq raw sequences of the V4 region 16S rRNA gene from the spleens of 190 bank voles (part 3)
Downloaded 42 times
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each individual using the MiSeq platform. The 190 multiplexed amplicons were indexed using both forward and reverse indexes. The list of the 190 multiplexed samples and associated indexes are provided in the following CSV file titled: Information concerning the samples multiplexed in the MiSeq run.
Download MiSeq_Reads_16Sv4_Myodes_part3.zip (291.1 Mb)
Details View File Details
Title Information concerning the samples multiplexed in the MiSeq run
Downloaded 11 times
Description This CSV file contains the sample names, the forward and reverse index names and the forward and reverse index sequences for each of the 190 samples multiplexed in the Illumina MiSeq run.
Download MiSeq_Reads_16Sv4_Myodes.csv (8.971 Kb)
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Title Contigs of sequences of the HiSeq RNA sequencing from the spleens of 190 bank voles
Downloaded 11 times
Description This FASTA file contains 5,338 contigs of sequences produced using HiSeq2000 RNAseq sequencing. These contigs result from the de novo assembly of HiSeq reads. They were assigned to bacteria via successive sequence alignment using the non-redundant nucleotide and protein databases from NCBI and the BLAST algorithm.
Download HiSeq_Contigs_RNAseq_bacteria_Myodes.fasta (1.350 Mb)
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When using this data, please cite the original publication:

Razzauti M, Galan M, Bernard M, Maman S, Klopp C, Charbonnel N, Vayssier-Taussat M, Eloit M, Cosson J (2015) A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife. PLOS Neglected Tropical Diseases 9(8): e0003929. http://dx.doi.org/10.1371/journal.pntd.0003929

Additionally, please cite the Dryad data package:

Razzauti M, Galan M, Bernard M, Maman S, Klopp C, Charbonnel N, Vayssier-Taussat M, Eloit M, Cosson J (2015) Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.50125
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