Data from: Spatial structure of ecological opportunity drives adaptation in a bacterium

 

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Title population fitness
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Description Relative fitness measures over time of Pseudomonas fluorescens SBW25 populations evolving in environments containing mannose, glucose, xylose, and all three sugars. Fitness measures are estimates from 24 hour competitions against the ancestor.
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Title genotype fitness
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Description Relative fitness of genotypes isolated from evolving populations of Pseudomonas fluorescens SBW24 every 200 generations for 1000 generations, assayed in mannose, glucose and xylose. Fitness measures are estimated from 24 hour competitions against the ancestor.
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Title BIOLOG growth
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Description Growth measures from BIOLOG assays of genotypes isolated from Pseudomonas fluorescens SBW25 populations evolved for 1000 generations in mannose, glucose, xylose, and the combination of all three sugars. Growth was measured as the difference between final and initial optical density (after 24 hours) in each well a BIOLOG GN2 plate. BIOLOG GN2 plates are comercially available 96-well plates containing different 95 different carbon sources plus a carbon-absent control well. A1 - H12 indicate well.
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Title ancestor fitness
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Description Pseudomonas fluorescens SBW25:lacZ+ fitness relative to Pseudomonas fluorescens SBW25:lacZ- measured on each selection environment used in our evolution experiment. Fitness was estimated via 24 hour competitions.
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Title replicated population fitness
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Description Additional replicated population fitness data for select evolved Pseudomonas fluorescens SBW25 lines. These fitness measures are a replication of some of the data in "population fitness.xls".
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Title Nal mutant fitness
Downloaded 53 times
Description Difference between fitness of Pseudomonas fluorescens SBW25 naladixic acid-resistant mutants and Pseudomonas fluorescens SBW25, measured in mannose, glucose, and xylose environments. Fitness differences were estimated from 24 hour competitions in three replicate assays.
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Title population density
Downloaded 122 times
Description Population density of Pseuodomonas fluorescens SBW25 populations evolving in environments containing mannose, glucose, xylose, and a combination of all three sugars over 1000 generations. Population density was measured by counting colony forming units after 24 hours of growth in the selection environment every 30 - 100 generations.
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When using this data, please cite the original publication:

Bailey SF, Kassen R (2012) Spatial structure of ecological opportunity drives adaptation in a bacterium. The American Naturalist 180(2): 270-283. http://dx.doi.org/10.1086/666609

Additionally, please cite the Dryad data package:

Bailey SF, Kassen R, Bailey SF, Kassen R (2012) Data from: Spatial structure of ecological opportunity drives adaptation in a bacterium. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.51mg4125
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