Data from: Effects of plant diversity on the concentration of secondary plant metabolites and the density of arthropods on focal plants in the field

Kostenko O, Mulder PPJ, Courbois M, Bezemer TM

Date Published: November 29, 2016

DOI: http://dx.doi.org/10.5061/dryad.54ht3

 

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Title Surrounding plant community and soil measurements
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Description A detailed description of the field experiment is presented in the article. At the end of August 2011, plant community measurements were made in each plot, to estimate the structural complexity of the community. For each plant community, the percentage cover of plant species was recorded in two 1 m2 quadrants along a diagonal transect within each plot. The total percentage cover can exceed 100% because plants in a community can overlap. The height of the vegetation was measured using the vertical drop disc method (Stewart et al. 2001). The disc weighed 200 g, had a diameter of 300 mm, and was released from a 1.5-m height. The height was measured at 10 random locations within each plot. One week after plant sampling, soil cores of 15 cm depth and 2.5 cm diameter were collected from each experimental plot at five random positions. The soil samples were pooled per plot and used for chemical analysis. Mineral N content (NH4+ and NO3) in soil samples was determined colorimetrically in the CaCl2 extraction using a Traacs 800 autoanalyser (TechniCon Systems Inc, USA). The C:N-ratio in soil samples was measured on a FlashEA 1112 Series NC soil analyser (Thermo Scientific). pH was measured in 2:5 dry soil : water suspensions. The percentage organic C was determined according to Nelson and Sommers (1982) and available P according to Olsen et al. (1954) and measured at 720 nm.
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Title Arthropod database 2011
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Description A detailed description of the field experiment is presented in the article. Arthropods at all stages of their development (eggs, immature and adults) on each Jacobaea vulgaris plant were collected on four occasions from May to August 2011. During each collection, all plants were carefully inspected between 10:00 am and 16:00 pm and all arthropods that were observed on a plant were collected using an aspirator by three collectors distributed evenly over the field. Each collector inspected all 1324 plants, spending an approximately equal amount of time at each plant at all diversity levels. All arthropods were stored individually in 70% ethanol in labelled Eppendorf tubes. Most arthropods were identified to species or family level. All arthropod species were assigned to feeding group (specialist herbivore, generalist herbivore, predator, pollinator, detritivore and omnivore) based on their feeding strategy and the degree of specialization.
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Title PA content J. vulgaris
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Description PA analysis of leaf and root samples was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) following the procedure outlined in Kostenko et al. (2013). In brief, 5 mg of freeze-dried ground plant material was extracted with 0.5 ml 2% formic acid solution containing heliotrine (1 µg•ml-1) as internal standard. After centrifugation and filtration, 25 µl of the extracted filtrate was diluted with 975 µl of 10 mM ammonium hydroxide solution and 10 µl was injected in a Waters Acquity ultra-performance chromatographic system coupled to a Waters Quattro Premier tandem mass spectrometer (Waters, Milford, MA, USA). Separation and mass spectrometric detection of the PAs was as described in Cheng et al. (2011) and Appendix S2. Data were processed using Masslynx 4.1 software.
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Title Jacobaea vulgaris_characteristics 2011
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Description A detailed description of the field experiment and plant measurements are presented in the article. Leaf carbon (C) and nitrogen (N) concentrations were determined using a Flash EA1112 CN analyser (Interscience, Breda, The Netherlands).
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When using this data, please cite the original publication:

Kostenko O, Mulder PPJ, Courbois M, Bezemer TM (2017) Effects of plant diversity on the concentration of secondary plant metabolites and the density of arthropods on focal plants in the field. Journal of Ecology 105(3): 647–660. http://dx.doi.org/10.1111/1365-2745.12700

Additionally, please cite the Dryad data package:

Kostenko O, Mulder PPJ, Courbois M, Bezemer TM (2016) Data from: Effects of plant diversity on the concentration of secondary plant metabolites and the density of arthropods on focal plants in the field. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.54ht3
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