Data from: Phosphatidylthreonine and lipid-mediated control of parasite virulence

Arroyo-Olarte RD, Brouwers JF, Kuchipudi A, Helms JB, Biswas A, Dunay IR, Lucius R, Gupta N

Date Published: November 20, 2015

DOI: http://dx.doi.org/10.5061/dryad.564sc

 

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Title X1_WT (Fig 4A)
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Title X1_KO (Fig 4B
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Title X1_compl (Fig 4C)
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Title MS and MS2 of PtdThr in T. gondii
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Description Negative ion mode MS (ion trap) and MS2 data collected during the elution of PtdThr. (Fig 1B & 1C).
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Title Base peak chromatogram (Fig1A)
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Description Base peak chromatogram (Fig1A) corresponding to the elution profile of lipids isolated from extracellular tachyzoites.
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Title Ion chromatogram of the separation of phospholipids from human fibroblasts (FigS2A)
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Description Ion chromatogram of the separation of phospholipids from human fibroblasts
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Title Mass spectra recorded at the elution time of PtdThr (FigS2B)
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Description In human fibroblasts, no PtdThr related ions are detected at the retention time PtdThr from T. gondii eluted. Detected ions are PtdSer species.
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Title Intracellular parasites can synthesize PtdThr using free threonine in cultures. (FigS14A)
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Description MS and MS2 data of PtdThr species
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Title Intracellular parasites can synthesize PtdThr using free threonine in cultures. (FigS14B)
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Description MS and MS2 data showing the incorporation of U-13C Threonine incorporation into PtdThr.
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When using this data, please cite the original publication:

Arroyo-Olarte RD, Brouwers JF, Kuchipudi A, Helms JB, Biswas A, Dunay IR, Lucius R, Gupta N (2015) Phosphatidylthreonine and lipid-mediated control of parasite virulence. PLOS Biology 13(11): e1002288. http://dx.doi.org/10.1371/journal.pbio.1002288

Additionally, please cite the Dryad data package:

Arroyo-Olarte RD, Brouwers JF, Kuchipudi A, Helms JB, Biswas A, Dunay IR, Lucius R, Gupta N (2015) Data from: Phosphatidylthreonine and lipid-mediated control of parasite virulence. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.564sc
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