Data from: Fluorescent protein-mediated colour polymorphism in reef corals: multi-copy genes extend the adaptation/acclimatization potential to variable light environments

Gittins JR, D'Angelo C, Oswald F, Edwards R, Wiedenmann J

Date Published: December 11, 2014

DOI: http://dx.doi.org/10.5061/dryad.5d079

 

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Title Abs spectra of A millepora
Downloaded 33 times
Description Absorption spectra of clarified tissue extracts of the HR and LR A. millepora morphs. The absorption spectrum of purified recombinant amilFP597 normalised to the peak value of the HR spectrum is included for comparison.
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Title amilFP597 copies
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Description Copy numbers of amilFP597 variant genes determined by semi-quantitative PCR amplification of a conserved genomic exon 3 fragment and by diagnostic restriction analysis with ApeK1.
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Title amilFP597 indel (+) and indel (-) promoter variants
Downloaded 29 times
Description Sequences of all promoter clones of amilFP597 coding sequence.
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Title amilFP597 exon 3 sequences
Downloaded 35 times
Description The chromophore coding sequences of RFP-related genes of A. millepora were amplified using genomic DNA of LR, MR and HR morphs as the template with primers designed to conserved regions within exon 3 .
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Title amilFP597 promoter
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Description Analysis of the amilFP597 promoter copy ratios in six A. millepora morphs with different levels of redness.
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Title Sequences RFP full length genes
Downloaded 38 times
Description All raw sequences used to reconstruct the amilFP597 gene in A. millepora.Full-length sequences of indel (-) and indel (+) promoter variants of the amilFP597 gene were obtained for the MR morph. These sequences, extending from the promoter region to the 3’UTR, were produced by joining the sequences of two overlapping PCR products covering the 5’ region [promoter to exon 3 amplified using primers pRFPlargeF (+) or pRFPsmallF (-) and RFP SP1] and the 3’ region (intron 2 to 3’UTR amplified using primers RFP_I2-3’U_F and RFP_I2-3’U_R2). Sequence differences in the overlap between the 5’ and 3’ region fragments were used to assign the latter to either the indel (+) or indel (-) promoter variant genes. The promoter-exon 3 and intron 2-3’UTR fragments were cloned and sequenced on both strands using vector primers and by primer walking. The assembled sequences have been submitted to GenBank as accessions KC818413 [indel (-) gene] and KC818414 [indel (+) gene].
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Title Spectroscopic characteristics of GFP-like proteins
Downloaded 37 times
Description All spectroscopic data utilised for the characterisation of amilFP597, amilCP506, amilCP564 and amilFP605
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When using this data, please cite the original publication:

Gittins JR, D'Angelo C, Oswald F, Edwards R, Wiedenmann J (2015) Data from: Fluorescent protein-mediated colour polymorphism in reef corals: multi-copy genes extend the adaptation/acclimatization potential to variable light environments. Molecular Ecology 24(2): 453-465. http://dx.doi.org/10.1111/mec.13041

Additionally, please cite the Dryad data package:

Gittins JR, D'Angelo C, Oswald F, Edwards R, Wiedenmann J (2014) Data from: Fluorescent protein-mediated colour polymorphism in reef corals: multi-copy genes extend the adaptation/acclimatization potential to variable light environments. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.5d079
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