Data from: Evolution of behavioral and cellular defenses against parasitoid wasps in the Drosophila melanogaster subgroup

Lynch ZR, Schlenke TA, de Roode JC

Date Published: February 12, 2016

DOI: http://dx.doi.org/10.5061/dryad.5t5m4

 

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Title Figure 1, phylogenetic tree
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Description Nexus file executable in MrBayes that was used to generate the tree from aligned and trimmed Amyrel sequences (GenBank accession numbers in the Supporting Information). Tree file created in FigTree from the resulting 50% majority rule consensus phylogeny.
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Title Figure 2, cellular immunity
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Description For all replicates of every fly-wasp species combination, number of dissected larvae that were attacked (contained a parasitoid egg), unattacked, or multiply attacked (contained >1 parasitoid egg). Number of surviving larvae that were moved to food vials to measure eclosion success (larvae_moved), which involved four possible outcomes: (i) fly emerges without a melanized capsule (unattacked_fly), (ii) fly emerges with a melanized capsule (fly_survived), (iii) wasp emerges (wasp_survived), or (iv) both insects die (death). Proportional eclosion outcomes (ii)-(iv) (fly_success, wasp_success, and death_rate) were calculated after subtracting unattacked_fly from larvae_moved, such that unattacked fly larvae were ignored in the analyses.
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Title Figure 3, oviposition maintenance
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Description Number of eggs laid over 5-day forced co-habitation assays by flies in vials without wasps (ctrl), with L. boulardi (Lb17), and with L. heterotoma (Lh14), where each row represents a vial. For each day (#1-5), f# is the number of female flies alive at the beginning of the day, e# is the number of eggs laid by the end of the day, and c# is the cumulative number of eggs laid per female, i.e. c1 = e1/f1, c2 = c1 + e2/f2, c3 = c2 + e3/f3, etc. Sets of replicates for each fly species are arranged by date; control and wasp vials were run simultaneously in approximately equal numbers and multiple groups were run if <5 vials per treatment. day5_dOR = (c5 for each Lb17 or Lh14 replicate) / (mean c5 across control vials run for the same fly species at the same time). Oviposition maintenance indices in Fig. 3 are mean day5_dOR across all Lb17 or Lh14 replicates for each fly species.
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Title Figure 4, trade-offs
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Description Newick tree file (same as Fig. 1) and "infiles" for Lb17 and Lh14, with mean cellular immunity index in column 1 and mean oviposition maintenance index in column 2 for each fly species, used as inputs for the Contrast program in PHYLIP v. 3.69. Phylogenetically independent contrasts from the resulting "outfiles" are compiled into Fig4.csv: cellular immunity index contrasts (CI_Lb17 and CI_Lh14) and oviposition maintenance index contrasts (OMI_Lb17 and OMI_Lh14).
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Title Figure 5a, D. yakuba oviposition
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Description Number of eggs laid over a 6-day forced co-habitation assay by D. yakuba in vials without wasps (control), with L. boulardi (exp_Lb17), and with L. heterotoma (exp_Lh14). Number of females in each vial at the start of the experiment (none of them died during the experiment), eggs# = eggs laid on days #1-6, total_eggs = sum from eggs1:eggs6, eggs_per_female = total_eggs / females.
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Title Figure 5b, D. yakuba offspring survival
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Description Eggs laid by D. yakuba in the three parental treatments (control, exp_Lb17, and exp_Lh14) were transferred to Petri dishes, grown to second instar, then exposed to female L. boulardi. The larvae were moved to food vials to eclose and egg-to-adult survival was measured. capsule = adult flies that had melanized capsules and thus survived parasitism; no_capsule = adult flies that did not have capsules and thus were not attacked as larvae; total = capsule + no_capsule.
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Title Figure 6, sight and smell mutants
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Description Number of eggs laid over 5-day forced co-habitation assays by flies in vials without wasps (ctrl), with L. boulardi (LB17), and with L. heterotoma (LH14), where each row represents a vial. Four D. melanogaster strains are represented: Orco2 = smell-deficient mutant, GMRhidII = sight-deficient mutant, w1118 = white-eyed mutant, OregonR = wild-type. For each day (#1-5), e# is the number of eggs laid by the end of the day, f# is the number of female flies alive at the beginning of the day, and cu# is the cumulative number of eggs laid per female, i.e. cu1 = e1/f1, cu2 = cu1 + e2/f2, cu3 = cu2 + e3/f3, etc.
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Title Figure S1, melanogaster / simulans adaptive significance
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Description D. melanogaster and D. simulans offspring produced in embryo collection chambers without wasps (control) and with Lb17 or Lh14 (wasp) were grown to second instar, then exposed to wasps. fly_win = number of flies that survived with melanized capsules; wasp_win = number of wasps that survived; death = number of larvae that did not eclose.
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When using this data, please cite the original publication:

Lynch ZR, Schlenke TA, de Roode JC (2016) Evolution of behavioral and cellular defenses against parasitoid wasps in the Drosophila melanogaster subgroup. Journal of Evolutionary Biology 29(5): 1016–1029. http://dx.doi.org/10.1111/jeb.12842

Additionally, please cite the Dryad data package:

Lynch ZR, Schlenke TA, de Roode JC (2016) Data from: Evolution of behavioral and cellular defenses against parasitoid wasps in the Drosophila melanogaster subgroup. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.5t5m4
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