Data from: Cholera outbreaks (2012) in three districts of Nepal reveal clonal transmission of multi-drug resistant Vibrio cholerae O1

Dixit SM, Johura F, Manandhar S, Sadique A, Rajbhandari RM, Mannan SB, Rashid M, Islam S, Karmacharya D, Watanabe H, Sack RB, Cravioto A, Alam M

Date Published: July 16, 2014

DOI: http://dx.doi.org/10.5061/dryad.600nd

 

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Title Dendrogram showing genomic fingerprints of V. cholerae O1 strains isolated in Nepal in 2012.
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Description The fingerprint pattern in the gel was analyzed using a computer software package, Bionumeric (Applied Maths, Belgium). After background subtraction and gel normalization, the fingerprint patterns were typed according to banding similarity and dissimilarity as recommended by the manufacturer. , using the Dice similarity coefficient and unweighted-pair group method employing average linkage (UPGMA) clustering, by using pulsed-field gel electrophoresis (PFGE) images of NotI- restriction digested genomic DNA of the tested Nepalese V. cholerae strains; PFGE images of contemporary V. cholerae O1 El Tor strains from Haiti and Bangladesh, including El Tor reference strain N16961 were also included. The scale bar at the top (left) indicates similarity coefficient. The banding patterns and the similarity coefficient revealed 100% similarity for all the tested Nepalese, Haitian, and Bangladeshi V. cholerae strains included in the dendrogram, suggesting high level of clonal relatedness.
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When using this data, please cite the original publication:

Dixit SM, Johura F, Manandhar S, Sadique A, Rajbhandari RM, Mannan SB, Rashid M, Islam S, Karmacharya D, Watanabe H, Sack RB, Cravioto A, Alam M (2014) Cholera outbreaks (2012) in three districts of Nepal reveal clonal transmission of multi-drug resistant Vibrio cholerae O1. BMC Infectious Diseases 14:392. http://dx.doi.org/10.1186/1471-2334-14-392

Additionally, please cite the Dryad data package:

Dixit SM, Johura F, Manandhar S, Sadique A, Rajbhandari RM, Mannan SB, Rashid M, Islam S, Karmacharya D, Watanabe H, Sack RB, Cravioto A, Alam M (2014) Data from: Cholera outbreaks (2012) in three districts of Nepal reveal clonal transmission of multi-drug resistant Vibrio cholerae O1. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.600nd
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