Data from: An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii

Li X, Zhang R, Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC

Date Published: January 17, 2016

DOI: http://dx.doi.org/10.5061/dryad.60mk5

 

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Title Supplemental Data Set 1. Structural differences in the CC-4533 genome relative to reference and other strains.
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Title Supplemental Data Set 2. High-confidence genomic differences unique to CC-4533.
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Title Supplemental Data Set 3. Binary codes for plate super-pooling.
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Title Supplemental Data Set 4. Binary codes for colony super-pooling.
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Title Supplemental Data Set 5. Read counts of each flanking sequence in each super-pool.
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Title Supplemental Data Set 6. Validation of flanking sequence deconvolution in randomly selected mutants.
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Title Supplemental Data Set 7. Full library data: colony positions, insertion sites, deconvolution and LEAP-Seq summary, gene annotation.
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Title Supplemental Data Set 8. LEAP-Seq read details for each flanking sequence.
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Title Supplemental Data Set 9. Summary of PCR results to check the insertion sites of randomly-picked mutants from the mutant library.
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Title Supplemental Data Set 10. Statistically significant insertion hotspots and coldspots.
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Title Supplemental Data Set 11. Insertions in genes encoding lipid droplet proteins were characterized by PCR.
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When using this data, please cite the original publication:

Li X, Zhang R, Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell 28(2): 367-387. http://dx.doi.org/10.1105/tpc.15.00465

Additionally, please cite the Dryad data package:

Li X, Zhang R, Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) Data from: An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.60mk5
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