Data from: Vertical distribution of marine invertebrate larvae in response to thermal stratification in the laboratory

Daigle RM, Metaxas A

Date Published: November 19, 2013



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Title survival data
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Description We measured larval mortality for S. droebachiensis at 3 different temperatures (3, 10 and 21 °C) reared in 4-l culture jars (n = 3). Temperatures in the jars were maintained either by placing them in a water bath (3 and 21 °C) or in a temperature-controlled room (10 °C). These temperatures encompass ambient and the extreme temperatures used in the experimental thermoclines. Six-day old larvae (each replicate was from a single parental pair, and reared at 10 °C) were used for this experiment. To quantify mortality, 30 larvae from every treatment for each replicate were transferred to a Petri dish at 0, 24 and 48 h, and categorized as live if swimming was observed using a Nikon SMZ 1500 dissecting microscope. Data set includes jar (replicate 4-l cuture jars), temperature (°C), time (h), and the number of live and dead larvae from a sample of 30 larvae
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Title vertical distribution
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Description We manipulated both the temperature in the bottom water layer (B) and the temperature difference between layers (ΔT) in an orthogonal design. We used, for S. droebachiensis (n = 5): 4 levels of ΔT (0, 3, 6 and 12 °C) and 3 levels of B (3, 6 and 9 °C); for A. rubens (n = 4): 3 levels ΔT (0, 6 and 12 °C) and 2 levels of B (6 and 12 °C); and for A. irradians (n = 4): 3 levels of ΔT (0, 5 and 11 °C) and 2 levels of B (5 and 11 °C). These combinations of factors were chosen to represent the conditions in the local environment. Since there were up to 12 treatment combinations for a particular species and we only had 4 thermocline chambers, not all replicates could be run simultaneously. For S. droebachiensis, different cohorts (from unique parental pairs) were blocked in time and all treatments were completed in 3 randomized groups of 4 within 26 h. For the other species, all replicates of all treatments were conducted in randomized order with a single larval cohort (from multiple parental pairs) within 48 h. Due to limitations of the seawater system at the time, the surface water was ~ 20 °C rather than 21 °C in the treatment combination of ΔT = 12 °C and B = 9 °C for S. droebachiensis. Once the thermoclines were established, 50 ml of seawater containing 250–350 larvae were introduced to each chamber within 1 cm from the bottom of the observation compartment (filled with 0.45 μm-filtered seawater) by gently pouring into a funnel attached to a small tube (2-mm inner diameter). Before being introduced to the experimental chamber, larvae were acclimated to the respective bottom temperature for 15 min. Larval position was visually determined to the nearest cm at 5, 15, 30, 45, 60 and 90 min after introduction for S. droebachiensis and A. irradians, and at 10, 30, 60 and 90 min for A. rubens. Larvae in the entire 50 cm water columns were counted in < 2 min, making repeat counts of individual larvae highly unlikely. Data set includes experimental species, date, temperature of the top and bottom layers of the water column (°C), the temperature difference between layers (°C), time (min), and the number of larvae counted at each depth (cm)
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When using this data, please cite the original publication:

Daigle RM, Metaxas A (2011) Vertical distribution of marine invertebrate larvae in response to thermal stratification in the laboratory. Journal of Experimental Marine Biology and Ecology 409(1–2): 89–98.

Additionally, please cite the Dryad data package:

Daigle RM, Metaxas A (2011) Data from: Vertical distribution of marine invertebrate larvae in response to thermal stratification in the laboratory. Dryad Digital Repository.
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