Data from: Oscillatory signatures underlie growth regimes in Arabidopsis pollen tubes: computational methods to estimate tip location, periodicity and synchronization in growing cells

Damineli DSC, Portes MT, Feijó JA

Date Published: April 4, 2017

DOI: http://dx.doi.org/10.5061/dryad.6806c

 

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Title Ratiometric kymographs of growing pollen tubes
Description Kymographs of Arabidopsis pollen tubes growing in vitro, featuring two channels (YFP and CFP) of the genetically encoded ratiometric calcium probe YC3.6.
Download ratiometric_kymographs.zip (4.375 Mb)
Download README.txt (1.113 Kb)
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Title MetaMorph tracking of the pollen tube tip
Description Tracking and growth rate series of the of the same growing pollen tubes where the ratiometric kymographs where extracted, performed with the 'Track Object' routine in MetaMorph with 3 different ROI sizes.
Download MetaMorph_Tracking.zip (311.3 Kb)
Download README.txt (815 bytes)
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Title Synchronized oscillations in growth, H+ flux and intracellular Ca2+
Description Growth rate series from a tracking software (MetaMorph) is provided, together with a kymograph with fluorescence of a calcium reporter (Yellow Cameleon 3.6) and extracellular H+ flux measurements at the pollen tube tip from an ion-selective vibrating probe. A video with the tracking trace also used as midline to produce the kymograph is also included.
Download README.txt (2.568 Kb)
Download Sync.zip (1.336 Mb)
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When using this data, please cite the original publication:

Damineli DSC, Portes MT, Feijó JA (2017) Oscillatory signatures underlie growth regimes in Arabidopsis pollen tubes: computational methods to estimate tip location, periodicity, and synchronization in growing cells. Journal of Experimental Botany, online in advance of print. http://dx.doi.org/10.1093/jxb/erx032

Additionally, please cite the Dryad data package:

Damineli DSC, Portes MT, Feijó JA (2017) Data from: Oscillatory signatures underlie growth regimes in Arabidopsis pollen tubes: computational methods to estimate tip location, periodicity and synchronization in growing cells. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6806c
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