Data from: Amino acid change in an orchid desaturase enables mimicry of the pollinator’s sex pheromone

Sedeek KEM, Whittle E, Guthörl D, Grossniklaus U, Shanklin J, Schlüter P

Date Published: May 20, 2016

DOI: http://dx.doi.org/10.5061/dryad.698s5

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title RNA in situ hybridization of SAD2-clade desaturases in Ophrys flowers.
Downloaded 35 times
Description Additional data for Figure S1: RNA in situ hybridization of SAD2-clade desaturases (SAD1 and SAD2) in Ophrys flowers. (A) Overview illustration showing O. exaltata and O. sphegodes flowers from top and front along with the layout and direction of view of thin sections (starting at the base of the labellum on top toward the apex at the bottom). (B) Two thin sections of O. exaltata labellum, at 3 and 5 mm from the base (top) of the labellum. (C) From top to bottom, O. sphegodes labellum thin sections at 1, 3, 5, 7 and 10 mm from the base (top) of the labellum. The antisense panels show blackish signal in the epidermal layer of O. sphegodes that is clearly stronger than the brownish background signal observed in the sense controls. However, no such difference was observed between sense and antisense signals in O. exaltata epidermis. The difference between O. sphegodes and O. exaltata is consistent with previous gene expression analyses [1, 2] that show SAD2-clade genes to be highly expressed in mature O. sphegodes labellum, whereas expression in O. exaltata labellum was low. The higher level of SAD2 expression as compared to SAD1 expression in O. sphegodes [1, 2] suggests that ISH signal in panel (C) is primarily due to SAD2. References: 1. Xu et al. (2012). PLoS Genet. 8, e1002889. 2. Schlüter et al. (2011). Proc. Natl. Acad. Sci. USA 108, 5696-5701.
Download ISH-data-1_Sph-SAD2+Exa-SAD2.pdf (807.8 Mb)
Details View File Details
Title RNA in situ hybridization of SAD3 in Ophrys flowers.
Downloaded 24 times
Description Additional data for Figure S1: RNA in situ hybridization of SAD3 (the only SAD3-clade member) in Ophrys flowers. The probes were hybridized (A) with four thin sections of O. exaltata at 1, 3, 5 and 7 mm and (B) with two sections of O. sphegodes at 5 and 7 mm from the base of the lip. Antisense signal was detected in the epidermal layer of both species as compared to the sense control, although this is much clearer in panel (A). Previous gene expression analyses showed SAD3 to be ubiquitously expressed in different plant tissues and developmental stages, suggesting it to encode a housekeeping desaturase [1, 2], and these expression data are consistent with ISH despite low signal intensity in panel (B). It should be noted that qualitative absence of clear antisense signal (e.g. in non-epidermal layers) cannot be taken as evidence of absence of SAD3 expression there. References: 1. Xu et al. (2012). PLoS Genet. 8, e1002889. 2. Schlüter et al. (2011). Proc. Natl. Acad. Sci. USA 108, 5696-5701.
Download ISH-data-2_Sph-SAD3+Exa-SAD3.pdf (1.807 Gb)
Details View File Details
Title RNA in situ hybridization of SAD5-clade desaturases in Ophrys flowers.
Downloaded 4 times
Description Additional data for Figure S1: RNA in situ hybridization of SAD5-clade desaturases (SAD5 and SAD6) in Ophrys flowers. Panel (A) shows thin sections of two individuals of O. exaltata and (B) one O. sphegodes individual; for each individual, two sections at 5 and 7 mm from the base (top) of the labellum were analysed. SAD5 antisense probe and sense control are shown for O. sphegodes. The antisense panels show black signal in the epidermal layer of O. exaltata (panel A), but not in O. sphegodes (panel B); O. exaltata antisense signal (panel A) is clearly stronger than the brownish background seen with the sense and antisense probes in O. sphegodes (panel B). This is consistent with the expression pattern reported for SAD5 [1], which is highly expressed in O. exaltata but not in O. sphegodes. References: 1. Xu et al. (2012). PLoS Genet. 8, e1002889.
Download ISH-data-3_Exa-SAD5+Sph-SAD5.pdf (1.070 Gb)
Details View File Details

When using this data, please cite the original publication:

Sedeek KEM, Whittle E, Guthörl D, Grossniklaus U, Shanklin J, Schlüter P (2016) Amino acid change in an orchid desaturase enables mimicry of the pollinator’s sex pheromone. Current Biology 26(11): 1505–1511. http://dx.doi.org/10.1016/j.cub.2016.04.018

Additionally, please cite the Dryad data package:

Sedeek KEM, Whittle E, Guthörl D, Grossniklaus U, Shanklin J, Schlüter P (2016) Data from: Amino acid change in an orchid desaturase enables mimicry of the pollinator’s sex pheromone. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.698s5
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: