Data from: Neuronal connectome of a sensory-motor circuit for visual navigation

Randel N, Asadulina A, Bezares-Calderón LA, Verasztó C, Shahidi R, Jékely G

Date Published: June 10, 2014

DOI: http://dx.doi.org/10.5061/dryad.6f267

 

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Title High-resolution image stack of the Platynereis larval primary optic neuropil
Downloaded 14 times
Description High-resolution (1.13 nm/pixel) image stack (tiff series) of the Platynereis larval primary optic neuropil. Contains 4 layers of 22,304 x 5680 pixels.
Download Dryad_dataset1.tif (506.7 Mb)
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Title acetylated tubulin staining registered to the unbiased nuclear reference
Downloaded 7 times
Description Tiff stack of average acetylated tubulin staining registered to the unbiased nuclear reference of 3-day-old Platynereis larvae. The antibody labels cilia and the axonal scaffold (white).
Download Dryad_dataset2.tif (99.45 Mb)
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Title Average full-body gene expression patterns of r-opsin1, hdc and VAChT
Downloaded 3 times
Description RGB tiff stack of average registered full-body gene expression patterns of eye marker gene r-opsin1 (red) and neurotransmitter marker genes hdc (green) and VAChT (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands, parapodia and chaetae of larvae is background.
Download Dryad_dataset3.tif (298.2 Mb)
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Title Average full-body gene expression patterns of VGluT, TrpH and ChAT
Downloaded 5 times
Description RGB tiff stack of average registered full-body gene expression patterns of neurotransmitter marker genes VGluT (red), TrpH (green) and ChAT (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands, parapodia and chaetae of larvae is background.
Download Dryad_dataset4.tif (298.2 Mb)
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Title Average full-body gene expression patterns of gad, TyrH and dbh
Downloaded 3 times
Description RGB tiff stack of average registered full-body gene expression patterns of neurotransmitter marker genes gad (red), TyrH (green) and dbh (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands and chaetae of larvae is background, however, signal in the parapodia in the TyrH (green) channel represents real gene expression.
Download Dryad_dataset5.tif (298.2 Mb)
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Title Double in situ hybridization for r-opsin1 and hdc
Downloaded 8 times
Description RGB tiff stack of raw data from high-resolution apical scan of double RNA whole mount in situ hybridization with r-opsin1 (red) and hdc (green) in 3-day-old Platynereis larvae. Larva is counterstained with acetylated tubulin antibody (blue) to show cilia and axonal scaffold.
Download Dryad_dataset6.tif (65.28 Mb)
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Title Blender file containing the complete virtual anatomical atlas of the Platynereis eye circuitry
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Description Simplified 3D models of all neurons, synapses, muscles and ciliated cells are included. The file can be viewed with the Blender software http://www.blender.org/.
Download Dryad_dataset7.blend (465.8 Mb)
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Title Node ranking in the Platynereis eye circuitry
Downloaded 31 times
Description Node ranking in the Platynereis eye circuitry based on various centrality measures.
Download Dryad_dataset8.xlsx (53.41 Kb)
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Title 3-day-old nuclear-stain whole-body reference template
Downloaded 3 times
Description Nuclear-stain whole-body reference template for 3-day-old (72 hours post fertilization) Platynereis larvae (tiff image stack). The whole-body nuclear-stain reference template was generated by the iterative registration of 40 individual scans.
Download Dryad_dataset9.tiff (99.57 Mb)
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Title Phototaxis ImageJ Macro
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Description ImageJ macro used to score phototaxis videos.
Download Dryad_dataset10.ijm (6.464 Kb)
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Title Phototaxis videos of control and mecamylamine-treated larvae
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Description Videos of phototaxis experiments. 3-day-old control larvae and larvae treated with 50 μm mecamylamine were imaged with 15 frames per second in a split phototaxis assay cuvette. The length of the cuvette is 15 mm. Directional white stimulus light was provided from the left. Each video is 600 frames. The videos are in Zeiss zvi format. The files were compressed with tar -jcvf archive_name.tar.bz2 directory_with_files/*, use tar –jxvf to uncompress them.
Download Dryad_dataset11.tar.bz2 (258.0 Mb)
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When using this data, please cite the original publication:

Randel N, Asadulina A, Bezares-Calderón LA, Verasztó C, Williams EA, Conzelmann M, Shahidi R, Jékely G (2014) Neuronal connectome of a sensory-motor circuit for visual navigation. eLife 3: e02730. http://dx.doi.org/10.7554/eLife.02730

Additionally, please cite the Dryad data package:

Randel N, Asadulina A, Bezares-Calderón LA, Verasztó C, Shahidi R, Jékely G (2014) Data from: Neuronal connectome of a sensory-motor circuit for visual navigation. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6f267
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