Data from: Development and evaluation of a fluorescent antibody-drug conjugate for molecular imaging and targeted therapy of pancreatic cancer

Knutson S, Raja E, Bomgarden R, Nlend M, Chen A, Kalyanasundaram R, Desai S

Date Published: June 29, 2016

DOI: http://dx.doi.org/10.5061/dryad.6j018

 

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Title Conjugate Characterization - Data and Graphs
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Description This file contains HPLC chromatograms and compiled numerical data concerning the characterization (drug:ab and fluorophore:ab ratios) of conjugates synthesized in this study (Fig 1 and Table 1).
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Title Conjugate Characterization - Linker Stability
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Description This file contains all HPLC chromatograms, graphs and computations for determining antibody-drug linker stability of the fluorescent antibody drug conjugate as described in the paper (Fig 2).
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Title In Vitro Fluorescent Evalution - Quantitation
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Description This file contains all of the raw and analyzed fluorescent quantitation data for assessing localization of conjugates with various cell lines (Fig. 3).
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Title Immunofluorescence Sample Image BxPC-3 CEA 680 PTX
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Description This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A.
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Title Immunofluorescence Sample Image BxPC-3 CEA 680
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Description This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A.
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Title Immunofluorescence Sample Image BxPC3 IgG 680 PTX
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Description This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A.
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Title Immunofluorescence Sample Image BxPC3 IgG 680
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Description This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A.
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Title In Vitro Fluorescent Evaluation - BxPC3 Flow Data
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Description This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4.
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Title In Vitro Fluorescent Evaluation - HeLa Flow Data
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Description This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4.
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Title In Vitro Fluorescent Evaluation - HepG2 Flow Data
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Description This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4.
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Title In Vitro Fluorescent Evaluation - MCF7 Flow Data
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Description This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4.
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Title In Vitro Fluorescent Evaluation - CEA Internalization Data
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Description This file contains the compiled and processed data surrounding the quantitative estimation of CEA internalization (Fig 5, panel B).
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Title In Vitro Cytotoxicity Evaluation - MTT Assay Compiled Data
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Description This excel file contains all of the compiled MTT assay data, as well as graphs (Fig 6) and linear regression analysis for estimating IC50 (Table 2).
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Title In Vitro Cytotoxic Evaluation - Flow Cytometry Cell Cycle Analysis
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Description This file contains the raw flow cytometry cell cycle analysis data and analyzed images that were arranged for Fig 7. This file was originally a template that was included with the BD cell cycle analysis kit as described in the paper.
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Title In Vivo Evaluation - Tumor Growth and Auto Exposure Data
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Description This excel file contains all raw tumor growth measurements and graphs for in vivo efficacy evaluation (Fig 8). This file also contains the compiled exposure times generated for Fig 10.
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Title In Vivo Imaging Sample Image - Day 7 - White Light - Raw
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Description This file is a raw jpeg generated for Fig 9. It depicts imaging of a mouse in group 3 imaged under white light as described in the methods section.
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Title In Vivo Imaging Sample Image - Day 7 - Infrared - Raw
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Description This file is a raw jpeg generated for Fig 9. It depicts a mouse in group 3 imaged under an infrared filter set as described in the methods section.
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Title In Vivo Imaging Sample Image - Day 7 - Infrared - False Color
Downloaded 18 times
Description This file is a jpeg generated for Fig 9. It depicts a mouse in group 3 imaged under an infrared filter as described in the methods section. Red false color has been added with Pxlr editor as described in the methods section.
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Title In Vivo Evaluation - ANOVA Analysis
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Description This file contains the statistical analysis of the data generated for Fig 8.
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When using this data, please cite the original publication:

Knutson S, Raja E, Bomgarden R, Nlend M, Chen A, Kalyanasundaram R, Desai S (2016) Development and evaluation of a fluorescent antibody-drug conjugate for molecular imaging and targeted therapy of pancreatic cancer. PLOS ONE 11(6): e0157762. http://dx.doi.org/10.1371/journal.pone.0157762

Additionally, please cite the Dryad data package:

Knutson S, Raja E, Bomgarden R, Nlend M, Chen A, Kalyanasundaram R, Desai S (2016) Data from: Development and evaluation of a fluorescent antibody-drug conjugate for molecular imaging and targeted therapy of pancreatic cancer. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6j018
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