The terrestrial worm Cognettia sphagnetorum has been used as a model in several studies focusing on research areas such as climate change as well as forest and soil ecology; it has also been shown to play a key role in the decomposition of organic matter and nutrient cycling. Cognettia is an enchytraeid genus commonly found in acidic terrestrial habitats, such as coniferous forests and bogs. In this study, the diversity of the genus, with particular focus on the morphospecies C. sphagnetorum in northern Europe, is assessed using four molecular markers, the mitochondrial COI (cytochrome c oxidase subunit I) and16S (16S ribosomal RNA), and the nuclear H3 (Histone 3) and ITS (Internal Transcribed Spacer). The datasets were first delimited into Molecular Operational Units (MOTUs) and the existence of global barcoding-gaps was tested. Single gene-trees were then estimated for all genes using Bayesian Inference, and a species tree was estimated with all markers combined using the multi-species coalescence. The results show that in northern Europe the genus consists of at least eight MOTUs supported by all genes except H3. Four of these MOTUs were within the morphotaxon C. sphagnetorum and two within Cognettia glandulosa. C. sphagnetorum s.l. was found to be non-monophyletic in all gene-trees, as well as in the species tree. As the MOTUs were well separated and non-monophyly was observed within C. sphagnetorum s.l., we conclude that the MOTUs are best treated as separate species. Given that cryptic diversity was found in this genus, we recommend that material of Cognettia used in future studies should be identified using molecular barcodes.
log file from *BEAST analysis
StarBEASTLog.log.txt
tree file from *BEAST analysis
StarBEASTLog.species.trees
xml input file for *BEAST analysis
StarBEASTLog.xml
Cognettia 16S consensus tree file from MrBayes analysis
16S_CognettiaMAFFT.nex.con
Cognettia COI consensus tree file from MrBayes analysis
COI_cognettia.nex.con
Cognettia H3 consensus tree file from MrBayes analysis
H3_Congettia.nex.con
Cognettia ITS consensus tree file from MrBayes analysis
ITS_CognettiaMAFFT.nex.con
Cognettia 16S MCMC file from MrBayes analysis
16S_CognettiaMAFFT.nex.mcmc
Cognettia COI MCMC file from MrBayes analysis
COI_cognettia.nex.mcmc
Cognettia H3 MCMC file from MrBayes analysis
H3_Congettia.nex.mcmc
Cognettia ITS MCMC file from MrBayes analysis
ITS_CognettiaMAFFT.nex.mcmc
Cognettia 16S nexus file for MrBayes analysis
16S_CognettiaMAFFT.nex
Cognettia COI nexus file for MrBayes analysis
COI_Cognettia.nex
Cognettia H3 nexus file for MrBayes analysis
H3_Congettia.nex
Cognettia ITS nexus file for MrBayes analysis
ITS_CognettiaMAFFT.nex
Cognettia 16S run1 p file from MrBayes analysis
16S_CognettiaMAFFT.nex.run1.p
Cognettia 16S run2 p file from MrBayes analysis
16S_CognettiaMAFFT.nex.run2.p
Cognettia COI run1 p file from MrBayes analysis
COI_cognettia.nex.run1.p
Cognettia COI run2 p file from MrBayes analysis
COI_cognettia.nex.run2.p
Cognettia H3 run1 p file from MrBayes analysis
H3_Congettia.nex.run1.p
Cognettia H3 run2 p file from MrBayes analysis
H3_Congettia.nex.run2.p
Cognettia ITS run1 p file from MrBayes analysis
ITS_CognettiaMAFFT.nex.run1.p
Cognettia ITS run2 p file from MrBayes analysis
ITS_CognettiaMAFFT.nex.run2.p
Cognettia 16S parts file from MrBayes analysis
16S_CognettiaMAFFT.nex.parts
Cognettia COI parts file from MrBayes analysis
COI_cognettia.nex.parts
Cognettia H3 parts file from MrBayes analysis
H3_Congettia.nex.parts
Cognettia ITS parts file from MrBayes analysis
ITS_CognettiaMAFFT.nex.parts
Cognettia 16S run1 t file from MrBayes analysis
16S_CognettiaMAFFT.nex.run1.t
Cognettia 16S run2 t file from MrBayes analysis
16S_CognettiaMAFFT.nex.run2.t
Cognettia COI run1 t file from MrBayes analysis
COI_cognettia.nex.run1.t
Cognettia COI run2 t file from MrBayes analysis
COI_cognettia.nex.run2.t
Cognettia H3 run1 t file from MrBayes analysis
H3_Congettia.nex.run1.t
Cognettia H3 run2 t file from MrBayes analysis
H3_Congettia.nex.run2.t
Cognettia ITS run1 t file from MrBayes analysis
ITS_CognettiaMAFFT.nex.run1.t
Cognettia ITS run2 t file from MrBayes analysis
ITS_CognettiaMAFFT.nex.run2.t
Cognettia 16S trprobs file from MrBayes analysis
16S_CognettiaMAFFT.nex.trprobs
Cognettia COI trprobs file from MrBayes analysis
COI_cognettia.nex.trprobs
Cognettia H3 trprobs file from MrBayes analysis
H3_Congettia.nex.trprobs
Cognettia ITS trprobs file from MrBayes analysis
ITS_CognettiaMAFFT.nex.trprobs