Data from: Metabarcoding and metabolome analyses of copepod grazing reveal feeding preference and linkage to metabolite classes in dynamic microbial plankton communities

Ray JL, Althammer J, Skaar KS, Simonelli P, Larsen A, Stoecker D, Sazhin A, Ijaz UZ, Quince C, Nejstgaard JC, Frischer M, Pohnert G, Troedsson C

Date Published: September 22, 2016

DOI: http://dx.doi.org/10.5061/dryad.6tn7c

 

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Title SRP076974
Description Sequence Read Archive containing sequence flowgram files for copepod gut content ("COP") and filterable plankton biomass from mesocosm seawater ("FIL") eukaryotic microbial communities. Sequences were generated from amplicon libraries generated using PCR primers targeting the V7 region of the eukaryote small subunit rRNA gene (SSU rRNA). Each flowgram file contains sequence information for 24 biological samples which were pooled and indexed prior to sequencing.
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Title Sequence read identifiers and CREST taxonomic assignments
Downloaded 4 times
Description This zipped archive contains 48 taxonomic assigment text files: one taxonomic assignment file (sequence identification number and taxonomy with tab separation) for each sample used in this study (24 copepod gut content samples and 24 filterable seawater plankton biomass samples). Taxonomic assignment of quality-trimmed sequences was performed using the CREST classifier with Silvamod reference database.
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Title AmpliconNoise OTU table
Downloaded 2 times
Description This table summarizes OTU (98% cut-off) abundance in all sequence samples used for this study. Rows of the table are OTUs and columns of the table are samples. Column names consist of "TYPE_TreatmentDate". TYPE indicates sample type ("COP" - copepod gut content or "FIL" - filterable seawater plankton biomass). Treatment designations indicate mineral nutrient additions for each treatment: "blank" - Control mesocosms that received no mineral nutrients, "NP" - mesocosms that received N + P addition, "NPSi" - mesocosms that received N + P + Si addition, "fjord" - samples taken from the adjacent Raunefjorden. Dates indicate sampling date in March 2012.
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Title Taxonomic assignments for AmpliconNoise OTUs
Downloaded 2 times
Description This file contains taxonomic assignments for AmpliconNoise-identified OTUs (98% cut-off). Taxonomic assignment of OTUs was performed using the CREST classifier with Silvamod reference database.
Download Ray_et_al-AmpliconNoise_OTUs_taxonomic_as...ts.txt (318.3 Kb)
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Title CREST taxonomic assignments table
Downloaded 1 time
Description This table shows the abundance of highest-rank CREST taxonomic assignments of sequence reads for all samples.
Download CREST_taxonomy_table.csv (128 Kb)
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When using this data, please cite the original publication:

Ray JL, Althammer J, Skaar KS, Simonelli P, Larsen A, Stoecker D, Sazhin A, Ijaz UZ, Quince C, Nejstgaard JC, Frischer M, Pohnert G, Troedsson C (2016) Metabarcoding and metabolome analyses of copepod grazing reveal feeding preference and linkage to metabolite classes in dynamic microbial plankton communities. Molecular Ecology 25(21): 5585-5602. http://dx.doi.org/10.1111/mec.13844

Additionally, please cite the Dryad data package:

Ray JL, Althammer J, Skaar KS, Simonelli P, Larsen A, Stoecker D, Sazhin A, Ijaz UZ, Quince C, Nejstgaard JC, Frischer M, Pohnert G, Troedsson C (2016) Data from: Metabarcoding and metabolome analyses of copepod grazing reveal feeding preference and linkage to metabolite classes in dynamic microbial plankton communities. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.6tn7c
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