Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.
HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 1 of 7.
Dorsal view of a fixed stage HH11PGK1:H2B-chFP embryo counterstained with DAPI collected using a 20x objective on a Zeiss 780 confocal microscope and Zen 2011 black edition 64 bit software. Total file size is 3.48GB. Part 1 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 2 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 3 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 4 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 5 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 6 of 7.
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HH11 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 7 of 7.
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Stage X PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 1 of 4
Dorsal view of an un-incubated stage X PGK1:H2B-chFP embryo counterstained with DAPI collected using a 20x objective on a Zeiss 780 confocal microscope and Zen 2011 black edition 64 bit software. Total file size is 2.07GB. Part 1 of 4.
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Stage X PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 2 of 4.
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Stage X PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 3 of 4.
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Stage X PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 4 of 4.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 1 of 7.
Dorsal view of a fixed stage HH5 PGK1:H2B-chFP embryo counterstained with DAPI collected using a 20x objective on a Zeiss 780 confocal microscope and Zen 2011 black edition 64 bit software. Total file size is 3.50GB. Part 1 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 2 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 3 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 4 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 5 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 6 of 7.
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HH5 PGK1:H2B-chFP Tiled Z Stack 20x.lsm. Part 7 of 7.
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