Data from: Seasonal changes in pollen limitation and femaleness along the snowmelt gradient in a distylous alpine herb, Primula modesta

Kameyama Y, Watanabe M, Kurosawa H, Nishimori T, Matsue D, Takyu M

Date Published: October 30, 2015

DOI: http://dx.doi.org/10.5061/dryad.74jt1

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title Number of flowers per plant
Downloaded 11 times
Description We set 14, 4, and 5 quadrats of the size 0.5 m × 0.5 m at the ridges, slopes, and valleys, respectively. During 2009 and 2010, we counted the number of flowering plants, flowers per plant, and total number of flowers within each quadrat, discriminating between morphs (pin and thrum).
Download Number of flowers per plant.xlsx (19.82 Kb)
Details View File Details
Title Number of plants
Downloaded 14 times
Description We set 14, 4, and 5 quadrats of the size 0.5 m × 0.5 m at the ridges, slopes, and valleys, respectively. During 2009 and 2010, we counted the number of plants within each quadrat.
Download Number of plants.xlsx (9.947 Kb)
Details View File Details
Title Pollinator visitation
Downloaded 10 times
Description We observed pollinator visits to the flowers of P. modesta during peak flowering at each site: early June, early July, and late July at the ridge, slope, and valley sites, respectively. Field observations were conducted during calm and fine periods during daylight hours (9:00 am–3:00 pm). The total observation times were 25 hours per 5 days, 12 hours per 3 days, and 14 hours per 4 days, at the ridge, slope, and valley sites, respectively. On each observation day, we arbitrary set 2 m × 2 m plot and recorded the pollinators and the number of flowers of P. modesta. Pollinators were classified into Diptera, Hymenoptera, Lepidoptera, and others during field observations, and major pollinators were collected and identified at the family or species level if possible.
Download Pollinator visitation.xlsx (41.48 Kb)
Details View File Details
Title Seed-set by experimental crosses
Downloaded 10 times
Description To estimate the potential ability to produce seeds at each site (ridge, slope, and valley), we conducted artificial pollination experiments within and nearby the quadrats over 2 years (2010 and 2011). The total numbers of pin and thrum plants pollinated were 78 and 77 during 2010 and 59 and 81 during 2011, respectively. The plants were covered with fine-meshed nylon nets to exclude insect visitors. For each plant, one target flower bud was emasculated just before opening, and other flowers were removed. After opening of the target flowers, hand pollination was conducted by legitimate pollen grains collected from a single pollen donor growing at least 5 m away from the recipient plant. All fruits derived from the pollination treatments were harvested just before dehiscence, and the number of seeds and undeveloped seeds or ovules were counted using a microscope.
Download Seed-set by experimental crosses.xlsx (22.27 Kb)
Details View File Details
Title Seed-set under natural conditions
Downloaded 6 times
Description To estimate the number of ovules and the seeds per fruit under natural conditions at each site (ridge, slope, and valley), we examined the plants growing within and nearby the quadrats over 2 years (2010 and 2011). The total number of pin and thrum plants examined was 78 and 79 during 2010 and 170 and 157 during 2011, respectively. We collected several fruits from each plant (2.8 on average) during 2010, and one fruit per plant during 2011. The total number of fruits collected from the pin and thrum plants was 233 and 204 during 2010 and 170 and 157 during 2011, respectively. The fruits were collected just before dehiscence, and the number of seeds and undeveloped seeds or ovules were counted using a microscope.
Download Seed-set under natural conditions.xlsx (46.92 Kb)
Details View File Details

When using this data, please cite the original publication:

Kameyama Y, Watanabe M, Kurosawa H, Nishimori T, Matsue D, Takyu M (2015) Seasonal changes in pollen limitation and femaleness along the snowmelt gradient in a distylous alpine herb, Primula modesta. Ecology and Evolution 5(22): 5352–5363. http://dx.doi.org/10.1002/ece3.1803

Additionally, please cite the Dryad data package:

Kameyama Y, Watanabe M, Kurosawa H, Nishimori T, Matsue D, Takyu M (2015) Data from: Seasonal changes in pollen limitation and femaleness along the snowmelt gradient in a distylous alpine herb, Primula modesta. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.74jt1
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: