Data from: De novo assembly and characterization of the transcriptome of the parasitic weed Cuscuta pentagona identifies genes associated with plant parasitism

 

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Title Supplemental_figures S1 - S7
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Description Supplemental Figure S1. Flow-chart showing steps in dodder transcriptome assembly and annotation, and downstream transcript clustering and differential expression analysis. Supplemental Figure S2. Transcript size distribution for Dodder_all_transcriptome. Supplemental Figure S3. Expression of non-annotated transcripts as detected by RT-PCR in dodder stems. Supplemental Figure S4. Pie charts for multilevel GO distribution of annotated transcripts in three categories: biological processes (A), cellular components (B) and molecular function (C). Supplemental Figure S5. Histogram representation of GOslim classification in three categories: biological processes (A), molecular function (B) and cellular components (C). Supplemental Figure S6. Distribution of transcripts annotated as enzymes among different enzyme classes. Supplemental Figure S7. Multidimensional scaling (MDS) plot of all replicates of each dodder tissue used for transcriptome assembly and, subsequently, transcript clustering and differential expression analysis.
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Title Supplemental_Tables I - III
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Description Supplemental Table I. Distribution of percent length coverage for the top matching UniProt database entries. Supplemental Table II. Size statistics and percentage of read mapping to annotated and un-annotated transcripts. Supplemental Table III. Primers used in RT-PCR analysis.
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Title Supplemental Dataset 1: Dodder_all_transcriptome.fa
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Description Supplemental Dataset 1. Sequences of all transcripts of Dodder_all_transcriptome in FASTA format. The file can also be downloaded as a FASTA file at http://de.iplantcollaborative.org/dl/e00e9ea8-6aad-439b-a1fa-49dd3939693d. The transcripts were named as Cpent_contig plus a serial number. Also included are the Trinity identifiers and length of each transcript in header description. These transcripts have also been deposited at DDBJ/EMB/GenBank under the accession GAON00000000.
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Title Supplemental Dataset 2: Dodder_final_transcriptome.fa
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Description Supplemental Dataset 2. Sequences of all transcripts of Dodder_final_transcriptome in FASTA format, obtained after filtering and clustering of transcripts from Dodder_all_transcriptome.fa as described in materials and methods. The FASTA file can be downloaded at http://de.iplantcollaborative.org/dl/a85e3682-4315-43e8-9f9d-597908616b4a. The Trinity identifiers and length of each transcript are included in header description.
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Title Supplemental Dataset 3: Dodder_predicted_CDS.fa
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Description Supplemental Dataset 3. Sequences of all predicted ORFs in FASTA format from Dodder_final_transcriptome. The FASTA file can also be downloaded at http://de.iplantcollaborative.org/dl/f3eb4e4a-05b8-4db4-94f2-87fb77c1622a. The ORFs were named as Cpent_putative_CDS plus a serial number. Also included are the Trinity identifiers and type of ORFs in header description.
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Title Supplementary Dataset 4. Annotation of transcripts form Dodder_final_transcriptome
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Description Supplemental Dataset 4. Combined annotation of dodder transcripts obtained from BLASTX against nr and TAIR10 database. Column titles: (1) Contig_id, (2) Trinity identifier, (3) length of contig, (4) sequence description from Blast2GO, (5) top hit sequence ID from nr database, (6) top hit gene ID from TAIR10, (7) top hit gene name from TAIR10 and (8) gene description from TAIR10.
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Title Supplementary Dataset 5: GO chart data
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Description Supplemental Dataset 5. GO chart data i.e. number of annotated transcripts in each GO-category along with GO-level, score and parent GO-terms for each category under biological process (sheet1), molecular function (sheet2) and cellular component (sheet3). Columns titles: (1) GO-level, (2) GO-term (Accession), (3) GO-term (Name), (4) Number of contigs belonging to the GO-term, (5) Score, (6) Parents GO-term (Accession) and (7) Parents GO-term (Name).
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Title Supplementary Dataset 6: GO-ids for annotated dodder transcripts
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Description Supplemental Dataset 6. GO-ids for all annotated dodder transcripts: all GO-ids (sheet1) and GO-slim ids (sheet2). Column titles: (1) Contig ID, (2) Trinity identifier and (3) GO-term (Accession).
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Title Supplementary Dataset 7: Enzyme code distribution from KEGG
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Description Supplemental Dataset 7. Enzyme code distribution from KEGG for all annotated dodder transcripts. Column titles: (1) Enzymatic Pathway, (2) Number of sequences in the pathway, (3) Enzyme class, (4) Enzyme ID/EC (Enzyme Code) number, (5) Number of annotated contigs in the class, (6) Trinity identifiers of the contigs and (7) Enzymatic Pathway ID.
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Title Supplementary Dataset 8: Normalized RSEM-estimated counts
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Description Supplemental Dataset 8. Normalized RSEM-estimated counts for all replicates of each dodder tissue used for transcript clustering and differential expression analysis. There were eight replicates for stems, prehaustoria, haustoria and flowers (four replicates each from dodder grown on two host plants, tomato and tobacco) and four replicates for seeds and seedlings.Column titles: (1) Contig ID, (2) Trinity identifier, (3 - 10) eight replicates of flowers, (11 - 18) eight replicates of haustoria, (19 - 26) eight replicates of prehaustoria, (27 - 30) four replicates of seedlings, (31 - 34) four replicates of seeds and (35 - 42) eight replicates of stem.
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Title Supplementary Dataset 9: Enriched GO-categories for PCA-SOM clusters
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Description Supplemental Dataset 9. Enriched GO-categories, both overall GO and GOslim, for all clusters generated from principal component analysis with self-organizing map. Cluster 1 – 12 are shown sequentially on sheet 1 – 12.
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Title Supplementary Dataset 10: Differentially expressed transcripts for each pair-wise comparison
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Description Supplemental Dataset 10. Differentially expressed transcripts (logFC " 1; FDR < 0.05) for each pair-wise comparison for all dodder tissues: flowers_vs_haustoria (sheet 1); flowers_vs_prehaustoria (sheet 2); flowers_vs_seedlings (sheet 3); flowers_vs_seeds (sheet 4); flowers_vs_stems (sheet 5); haustoria_vs_prehaustoria (sheet 6); haustoria_vs_seedlings (sheet 7); haustoria_vs_seeds (sheet 8); haustoria_vs_stems (sheet 9); prehaustoria_vs_seedlings (sheet 10); prehaustoria_vs_seeds (sheet 11); prehaustoria_vs_stems (sheet 12); seedlings_vs_seeds (sheet 13); seedlings_vs_stems (sheet 14) and seeds_vs_stems (sheet 15). Column titles: (1) Contig ID, (2) Trinity identifier, (3) logFC (FoldChange), (4) logCPM (Counts Per Million), (5) PValue, (6) FDR (False discovery rate), (7) Sequence description from Blast2GO, (8) Top hit sequence ID from nr database, (9) Top hit gene ID from TAIR10, (10) Top hit gene name from TAIR10 and (11) Top hit gene description from TAIR10.
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Title Supplementary Dataset 11: Up-regulated and down-regulated transcripts at prehaustorial stage
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Description Supplemental Dataset 11. Shared up-regulated and down-regulated transcripts at prehaustorial stage compared to seedlings and stems and associated enriched GO categories: up-regulated transcripts (sheet 1); down-regulated transcripts (sheet 2); enriched GO-terms in up-regulated transcripts (sheet3) and enriched GO-terms in downregulated transcripts (sheet4). Column titles for sheet 1 and sheet2: (1) Contig ID, (2) Trinity identifier, (3) Sequence description from Blast2GO, (4) gene name from TAIR10 and (5) gene description from TAIR10.
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Title Supplementary Dataset 12: Up-regulated and down-regulated transcripts at haustorial stage
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Description Supplemental Dataset 12. Shared up-regulated and down-regulated transcripts at haustorial stage compared to prehaustoria, seedlings and stems and associated enriched GO-categories: up-regulated transcripts (sheet 1); down-regulated transcripts (sheet 2); enriched GO-terms in up-regulated transcripts (sheet3) and enriched GO-terms in downregulated transcripts (sheet4). Column titles for sheet 1 and sheet2: (1) Contig ID, (2) Trinity identifier, (3) Sequence description from Blast2GO, (4) gene name from TAIR10 and (5) gene description from TAIR10.
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Title Supplementary Dataset 13. Enriched GO terms for differentially expressed transcripts
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Description Supplemental Dataset 13. GO terms enriched for shared transcripts upregulated or downregulated for each dodder developmental stage compared to all other stages: seeds (sheet 1); seedlings (sheet 2); stem (sheet 3); prehaustoria (sheet 4); haustoria (sheet4) and flowers (sheet 5).
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Title Supplementary Dataset 14: Non-annotated transcripts differentially expressed in dodder infective stages
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Description Supplemental Dataset 14. List of non-annotated transcripts showing differential expression (logFC " 1; FDR < 0.05) in prehaustorial and haustorial stages compared to seedlings and stems. Column titles: (1) Contig ID, (2) Trinity identifier, (3) length of contig and (4) ORF prediction.
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Title Supplementary Dataset 15: Transcripts underlying GO-terms transport (GO:0006810) and transporter (GO:0005215) showing increased expression in preahustoria and haustoria.
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Description Supplemental Dataset 15. Transcripts underlying GO-terms transport (GO:0006810) and transporter (GO:0005215) represented among upregulated genes in prehaustorial stage compared to seedlings and stems (sheet 1), and in haustorial stage compared to prehaustoria, seedlings and stems (sheet 2).
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When using this data, please cite the original publication:

Ranjan A, Ichihashi Y, Farhi M, Zumstein K, Townsley B, David-Schwartz R, Sinha NR (2014) De novo assembly and characterization of the transcriptome of the parasitic weed Cuscuta pentagona identifies genes associated with plant parasitism. Plant Physiology, online in advance of print. http://dx.doi.org/10.1104/pp.113.234864

Additionally, please cite the Dryad data package:

Ranjan A, Ichihashi Y, Farhi M, Zumstein K, Townsley B, David-Schwartz R, Sinha NR (2014) Data from: De novo assembly and characterization of the transcriptome of the parasitic weed Cuscuta pentagona identifies genes associated with plant parasitism. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.7fr20
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