Data from: Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response

Reid, William D. K., Newcastle University

Close, Andrew J., Newcastle University

Humphrey, Suzanne, University of Liverpool

Chaloner, Gemma, University of Liverpool

Lacharme-Lora, Lizeth, University of Liverpool

Rothwell, Lisa, University of Edinburgh

Kaiser, Pete, University of Edinburgh

Williams, Nicola J., University of Liverpool

Humprey, Thomas J., Newcastle University

Wigley, Paul, University of Liverpool

Rushton, Stephen P., University of Newcastle Australia

Humphrey, Tom J., Swansea University

Published Feb 16, 2016 on Dryad. https://doi.org/10.5061/dryad.7h3v1

Cite this dataset

Reid, William D. K. et al. (2016). Data from: Cytokine responses in birds challenged with the human food-borne pathogen Campylobacter jejuni implies a Th17 response [Dataset]. Dryad. https://doi.org/10.5061/dryad.7h3v1

Abstract

Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here, we show that broiler chickens mount a breed-dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, , IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed-dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonization of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds.

Usage notes

reid_openscience_data

These cytokine data are based on total RNA samples taken from caecal tissue of commercial broilers, which were held under laboratory conditions. The birds were challenged with the pathogen Campylobacter jejuni. The results are expressed as fold changes in corrected target gene expression in infected birds relative to controls.