Data from: BAC library construction, screening and clone sequencing of lake whitefish (Coregonus clupeaformis, Salmonidae) towards the elucidation of adaptive species divergence

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title CARB assembly (ace) file
Downloaded 27 times
Description De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for carboxylesterase (CARB). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484
Download CARB.ace (15.63 Mb)
Details View File Details
Title HSC assembly (ace) file
Downloaded 29 times
Description De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for heat shock cognate 70 kDa protein (HSC). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484
Download HSC.ace (31.88 Mb)
Details View File Details
Title MDH assembly (ace) file
Downloaded 18 times
Description De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for cytoplasmic malate dehydrogenase (MDH). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484
Download MDH.ace (9.257 Mb)
Details View File Details
Title GAPDH assembly (ace) file
Downloaded 20 times
Description De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484
Download GAPDH.ace (22.13 Mb)
Details View File Details
Title MHC assembly (ace) file
Downloaded 24 times
Description De novo assembly of pyrosequencing data (454 Genome Sequencer FLX System, with long-read GS FLX Titanium chemistry) for 2 pooled BAC clones targeted for MHC classII beta (MHC). GS De novo Assembler software (Roche, Basel, Switzerland) was used with the following parameters: large or complex genome option, heterozygosity option, trimming database of the pCC1BAC HindIII vector, screening database of the E. coli str. K12 substr. DH10B genome (GenBank [accession number CP000948]), minimum overlap length 200 bp, minimum overlap identity 98% and minimum contig length 500 bp. Raw data available at Sequence Read Archive at NCBI: SRP003484
Download MHC.ace (5.535 Mb)
Details View File Details

When using this data, please cite the original publication:

Jeukens J, Boyle B, Kukavica-Ibrulj I, St-Cyr J, Lévesque RC, Bernatchez L (2011) BAC library construction, screening and clone sequencing of lake whitefish (Coregonus clupeaformis, Salmonidae) towards the elucidation of adaptive species divergence. Molecular Ecology 11(3): 541–549. http://dx.doi.org/10.1111/j.1755-0998.2011.02982.x

Additionally, please cite the Dryad data package:

Jeukens J, Boyle B, Kukavica-Ibrulj I, St-Cyr J, Lévesque RC, Bernatchez L (2011) Data from: BAC library construction, screening and clone sequencing of lake whitefish (Coregonus clupeaformis, Salmonidae) towards the elucidation of adaptive species divergence. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.8100
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: