Data from: Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

Ali N, Heslop-Harrison JS, Ahmad H, Graybosch RA, Hein GL, Schwarzacher T

Date Published: April 14, 2016

DOI: http://dx.doi.org/10.5061/dryad.82f5q

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title WSMV disease scores raw data
Downloaded 9 times
Description Lines were originally scored in 2002 for response to natural infection by WSMV at Hays, KS USA and Sidney, NE USA (Divis et al., 2006). Visual subjective assessment of the degree of chlorosis and plant stunting were used (Table 1 and footnotes). Selected lines were further evaluated in the field at the University of Nebraska Agricultural Research and Development Center, Mead, NE. In 2011 individual spikes from each line were harvested and used to seed at least 20, 1.2 m replicate rows of single plant-derived lines in September 2011 as a block surrounded by early planted wheat that served as a ‘green bridge’ for the development of WCM populations. Multiple plantings of the resistant cultivar Mace and the susceptible line Tomahawk were included as a control
Download WSMV disease scores raw data.xlsx (43.32 Kb)
Details View File Details
Title PCR gels of markers in TableS1 and S2_I
Downloaded 34 times
Description First of two files with images of gels used for scoring.
Download PCR gels of markers in TableS1 and S2_I.pdf (2.669 Mb)
Details View File Details
Title PCR gels of markers in TableS1 and S2_II
Downloaded 30 times
Description Second of two files of gel images used for scoring.
Download PCR gels of markers in TableS1 and S2_II.pdf (1.251 Mb)
Details View File Details
Title FISH_Experiment
Downloaded 20 times
Description Table with FISH experiments analysed for each line described
Download FISH_Experiments.pdf (201.6 Kb)
Details View File Details
Title FISH_Experiment_Schedules_I
Downloaded 47 times
Description First of two files with slides and probes for FISH experiments
Download FISH_Experiment_Schedules_I.pdf (3.421 Mb)
Details View File Details
Title FISH_Experiment_Schedules_II
Downloaded 9 times
Description Second of two files with slides and probes for FISH experiments
Download FISH_Experiment_Schedules_II.pdf (1.097 Mb)
Details View File Details
Title FISH experiments of line Millenium
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download Millenium.zip (35.69 Mb)
Details View File Details
Title FISH experiments for line KS95H102
Downloaded 8 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download KS95H102.zip (133.0 Mb)
Details View File Details
Title FISH experiment for line KS96HW10-1
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download KS96HW10-1.zip (288.7 Mb)
Details View File Details
Title FISH experiments for line N02Y5003
Downloaded 8 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5003.zip (194.1 Mb)
Details View File Details
Title FISH Experiments for line N02Y5096
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5096.zip (47.92 Mb)
Details View File Details
Title FISH Experiments for line N02Y5018
Downloaded 2 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5018.zip (261.3 Mb)
Details View File Details
Title FISH experiments for line N02Y5019
Downloaded 2 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5019.zip (336.6 Mb)
Details View File Details
Title FISH experiments for N02Y5021
Downloaded 2 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5021.zip (173.5 Mb)
Details View File Details
Title FISH experiments for line N02Y5025
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5025.zip (303.6 Mb)
Details View File Details
Title FISH experiments for line N02Y5057
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5057.zip (282.2 Mb)
Details View File Details
Title FISH experiments for line N02Y5075
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorscence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5075.zip (117.2 Mb)
Details View File Details
Title FISH experiments for line N02Y5078
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5078.zip (241.6 Mb)
Details View File Details
Title FISH experiments for line N02Y5082
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5082.zip (493.3 Mb)
Details View File Details
Title FISH experiments for line N02Y5105
Downloaded 3 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5105.zip (131.7 Mb)
Details View File Details
Title FISH experiments for line N02Y5106
Downloaded 3 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5106.zip (392.1 Mb)
Details View File Details
Title FISH experiments for line N02Y5109
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5109.zip (524.1 Mb)
Details View File Details
Title FISH experiments for line N02Y5117 Mace
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5117 Mace.zip (333.1 Mb)
Details View File Details
Title FISH experiments for line N02Y5121
Downloaded 6 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5121.zip (149.3 Mb)
Details View File Details
Title FISH experiments for line N02Y5149
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5149.zip (360.0 Mb)
Details View File Details
Title FISH experiments for line N02Y5154
Downloaded 7 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5154.zip (187.3 Mb)
Details View File Details
Title FISH experiments for line N02Y5156
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y5156.zip (699.8 Mb)
Details View File Details
Title FISH experiments for line N02Y5163
Downloaded 3 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail
Download N02Y5163.zip (153.2 Mb)
Details View File Details
Title FISH experiments for line N02Y2016
Downloaded 5 times
Description Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination, see FISH experiment file for list and FISH schedule files for more detail.
Download N02Y2016.zip (930.4 Mb)
Details View File Details

When using this data, please cite the original publication:

Ali N, Heslop-Harrison JS, Ahmad H, Graybosch RA, Hein GL, Schwarzacher T (2016) Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance. Heredity 117(2): 114-123. http://dx.doi.org/10.1038/hdy.2016.36

Additionally, please cite the Dryad data package:

Ali N, Heslop-Harrison JS, Ahmad H, Graybosch RA, Hein GL, Schwarzacher T (2016) Data from: Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.82f5q
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: