Data from: Detection of airborne genetically modified maize pollen by real-time PCR

 

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Title 18S-16S rDNA clones from bioaerosol sample G4 (S4F4)
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Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G4. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
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Title 18S-16S rDNA clones from bioaerosol sample G4 (S4F3)
Downloaded 23 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G4. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S4F3.txt (8.988 Kb)
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Title 18S-16S rDNA clones from bioaerosol sample G6 (S6F1)
Downloaded 20 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G6. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S6F1.txt (8.058 Kb)
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Title 18S-16S rDNA clones from bioaerosol sample G6 (S6F2)
Downloaded 19 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G6. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S6F2.txt (8.687 Kb)
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Title 18S-16S rDNA clones from bioaerosol sample G6 (S6F3)
Downloaded 17 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G6. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S6F3.txt (9.082 Kb)
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Title 18S-16S rDNA clones from bioaerosol sample G4 (S4F2)
Downloaded 13 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G4. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S4F2.txt (9.041 Kb)
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Title 18S-16S rDNA clones from bioaerosol sample G4 (S4F1)
Downloaded 21 times
Description 18S-16S rDNA clones from a maize pollen enriched bioaerosol sample collected with a pollen trap (PMF/Sigma 2, TIEM Technique, Germany) in a coexistence field trial in the Ghent area, Belgium. Sequences obtained from sample G4. Primers used: 515f 5’-GTGCCAGCMGCCGCGGTAA-3’ (M=A-C) 1391r 5’-GACGGGCGGTGWGTRCA-3’(W=A-T; R=A-G).
Download S4F1.txt (9.018 Kb)
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Title GenBank accession numbers of the closest macthes to the sequences obtained from samples G4 and G6
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Description The file includes, for each sequence retrieved from G4 and G6, the GenBank accession number, the % identity and the species name of the best matching sequence. % identities were obtained by comparing G4 and G6 sequences against the NCBI nucleotide sequence database by using BLASTN (Altschul et al. 1997). Only when identity was higher than 97% genus and/or species identification was attempted.
Download GenBank accession numbers of the closest ...G6.txt (10.93 Kb)
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When using this data, please cite the original publication:

Folloni S, Kagkli D, Rajcevic B, Guimarães N, Valicente FH, Van Droogenbroeck B, Van den Eede G, Van den Bulcke M (2012) Detection of airborne genetically modified maize pollen by real-time PCR. Molecular Ecology Resources 12(5): 810-821. http://dx.doi.org/10.1111/j.1755-0998.2012.03168.x

Additionally, please cite the Dryad data package:

Folloni S, Kagkli D, Rajcevic B, Guimarães N, Valicente FH, Van Droogenbroeck B, Van den Eede G, Van den Bulcke M (2012) Data from: Detection of airborne genetically modified maize pollen by real-time PCR. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.82th6
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