BACKGROUND: Understanding of the genetic architecture of plant UV-B responses allows extensive targeted testing of candidate genes or regions, along with combinations of those genes, for placement in metabolic or signal transduction pathways. RESULTS: Composite interval mapping and single-marker analysis methods were used to identify significant loci for cotyledon opening under UV-B in four sets of recombinant inbred lines. In addition, loci important for canalization (stability) of cotyledon opening were detected in two mapping populations. One candidate locus contained the gene HY5. Mutant analysis demonstrated that HY5 was required for UV-B-specific cotyledon opening. CONCLUSIONS: Structured mapping populations provide key information on the degree of complexity in the genetic control of UV-B-induced cotyledon opening in Arabidopsis. The loci identified using quantitative trait analysis methods are useful for follow-up testing of candidate genes.
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Details about significant quantitative trait loci from the BayxSha mapping population.
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Details about significant quantitative trait loci from the ColxKas mapping population.
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Details about significant quantitative trait loci from the LerxCol mapping population.
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Details about significant quantitative trait loci from the LerxCvi mapping population.
Copy of 1471-2229-10-112-s5
All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate)
and -UV-B (Mylar).
Copy of 1471-2229-10-112-s6
All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate)
and -UV-B (Mylar).
Copy of 1471-2229-10-112-s7
Additional File 7: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate)
and -UV-B (Mylar).
Copy of 1471-2229-10-112-s8
Additional File 8: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate) and -UV-B (Mylar).
Copy of 1471-2229-10-112-s9
Additional File 9: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate) and -UV-B (Mylar).
Copy of 1471-2229-10-112-s10
Additional File 10: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate) and -UV-B (Mylar).
Copy of 1471-2229-10-112-s11
Additional File 11: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate) and -UV-B (Mylar).
Copy of 1471-2229-10-112-s12
Additional File 12: All measured cotyledon-opening angles, arranged by experimental round and RIL population in excel spreadsheet format (xls). Each file contains angles for with both +UV-B (cellulose di-acetate) and -UV-B (Mylar).
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Additional File 13: UV Spectra. Additional description and a figure showing the spectral output of the UV313 bulbs and the irradiance under the Mylar-D and cellulose di-acetate filters used for -UV-B and +UV-B treatment of cotyledons.