Data from: ‘Venus trapped, Mars transits’: Cu and Fe redox chemistry, cellular topography and in situ ligand binding in terrestrial isopod hepatopancreas

Kille P, Morgan AJ, Powell K, Mosselmans JFW, Hart D, Gunning P, Hayes A, Scarborough D, Mcdonald I, Charnock JM

Date Published: April 25, 2016

DOI: http://dx.doi.org/10.5061/dryad.8734c

 

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Title Fig2_RawData
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Description Figure 2. µXRF maps of element distributions in unstained methacrylate-embedded thin mid-tubule sections of woodlouse hepatopancreas. A. Light micrograph of a transverse section from a woodlouse sampled at 02:00 hours (i.e. the beginning of ‘B’-cell restitution during darkness). Note that the morphology of the sections used for ?XRF is relatively unclear; this is due to a lack of differential contrast in sections that were unstained and infiltrated with methacrylate resin. S5_sp_light3x10.tif B. Superimposed Cu, Fe and Zn µXRF maps of the section depicted in 2A; note the co-distribution of Cu and Zn in ‘S’-cells (arrow heads), and the distribution of diffuse Fe mainly in ‘B’-cell apical cytoplasm that might be undergoing blebbing (arrows), and some Fe signal within the tubule lumen (broken arrows). File Prefix 6191 C. Superimposed Cu, P and S µXRF maps of the section depicted in 2A; note the co-distribution of Cu and S in ‘S’-cells (arrowheads), and the mainly apical distribution of P in ‘B’-cells (arrows). File Prefix 6191 D. Light micrograph of a mid-tubule transverse section from a woodlouse at 12:00 hours (i.e. at the beginning of ‘B’-cell extrusion during the day). S4_sp_Light4x10 E. Superimposed Cu, Fe and Zn µXRF maps of the section depicted in 2D; note that there is a partial section of a second tubule in the top-right of the micrograph, and that the transition metal distribution patterns are similar to those seen in 2B. File prefix - 5983 F. Expanded view µXRF maps for the region delineated by a broken-lined rectangle in 2E; note that Fe is distributed above and below the nuclear plane (arrow) of the prominent ‘B’-cell, albeit mainly in the apical cytoplasm. File prefix - 5984 G and H are schematic diagrams derived from Hames and Hopkin [36] illustrating the gross difference between ‘B’- and ‘S’-cell morphology (G), and a ‘clock’ summarizing the diurnal cycle of apocrine secretion in ‘B’-cells in relation to the light:dark regime with our two XAS sampling points superimposed upon it (H). File types included: tif - Image files (Light Micrographs) .dat : map data showing the stage positions in mm and windowed fluorescence counts. Column format: Horizontal position ; vertical position; time per point (ms); I0; It; Idrain; mca file. .edf file ESRF format file for program PyMCA containing the summed MCA files for each point in a map row by row. Binary data format .rgb file windowed detector counts for selected chemical elements of interest , column format: y index; x index; time; i0 it idrain; then elements as listed in first row.
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Title Fig3_RawData
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Description Figure 3. Panel A. Cu K-edge XANES spectra from: O. asellus ‘S’-cells at 02:00 hours (red; i-iv) Spectral range 5960-65 (n=5) Spectral range 6085-89 (n=5) Spectral range 6099-103 (n=5) Spectral range 6123-27 (n=5) O. asellus 12:00 hours (green; v-vi); Spectral range 5993-98 (n=4) Spectral range 6174 (n=1) O. asellus ‘B’-cells at 02:00 hours (blue; vii-xi) Spectral range 5975-79 (n=5) Spectral range 6090-94 (n=5) Spectral range 6110-14 (n=5) Spectral range 6133-37 (n=5) Spectral range 6148-49 (n=2) O. asellus 12:00 hours (brown; xii) clock time Spectral range 6175 (n=1) see Table 2 for the fit data File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title Fig4_RawData
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Description Figure 4. A. Cu K-edge EXAFS data (solid lines) and fits (broken lines) from: O. asellus ‘S’-cell at 02:00 hours (red; i) Spectral Range 5960-65 (n=5) O. asellus ‘B’-cells at 02:00 hours (blue; ii & iii) Spectral Range 5975-79 (n=5) Spectral Range 6148-49 (n=2) O. asellus ‘B’-cells 12:00 hours (brown; iv) Spectral Range 6175 (n=2) (see Table 3 for corresponding quantitative data). B. Fourier transforms of the Cu EXAFS data (solid lines) and fits (broken lines) depicted in Fig. 4A. File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title Fig5_RawData
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Description Figure 5. A. Fe K-edge XANES spectra from: O. asellus ‘S’-cell at 12:00 hours (green; i) Spectral Range 6172 (n=1) O. asellus ‘B’-cells at 02:00 hours (blue; ii - iv) Spectral Range 5967-74 (n=8) Spectral Range 6104-08 (n=5) Spectral Range 6128-32 (n=5) O. asellus ‘B’-cells 12:00 hours (brown; v - vi) Spectral Range 5985-91 (n=7) Spectral Range 6170-71 (n=2) (see Table 2 for the fit data) File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title Fig6_RawData
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Description Figure 6. A. Fe K-edge EXAFS data (solid lines) and fits (broken lines) from: O. asellus ‘S’-cell at 12:00 hours (green; i) Spectral Range 6172 (n=1) O. asellus ‘B’-cells 02:00 hours (blue; ii) Spectral Range 5967-74 (n=7) O. asellus ‘B’-cells 12:00 hours (brown; iii & iv) Spectral Range 5985-91 (n=7) Spectral Range 6170-71 (n=2) (see Table 3 for corresponding quantitative data). B. Fourier transforms of the Fe EXAFS data (solid lines) and fits (broken lines) depicted in Fig. 6A. File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title Fig7_RawData
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Description Figure 7. Radiation sensitivity of Cu assessed by collecting a sequential series of 5 XANES spectra from given regions in 3 different ‘B’-cells (panels A, B, C). The thin methacrylate-embedded sections were obtained from a woodlouse sampled at 02:00 hours. In each panel, the large down-pointing vertical arrow indicates that the top spectrum was collected first and the lowest spectrum last. Note that the shoulder on the absorption edge at 8982.5 eV (broken arrows), whilst remaining visible, is progressively eroded from the third acquired spectrum onwards. Panel A Spectral Range 6110-14 (n=5) Panel B Spectral Range 6090-94 (n=5) Panel C Spectral Range 6133-37 (n=5) File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title Fig8_RawData
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Description Figure 8. Radiation sensitivity of Fe assessed by collecting a sequential series of 5 XANES spectra from given regions in a ‘B’-cell (panel A) Spectral Range 6104-08 (n=5) ‘S’-cell (panel B) Spectral Range 6128-32 (n=5) The thin methacrylate-embedded sections were obtained from a woodlouse sampled at 02:00 hours. In both panels, the large down-pointing vertical arrow indicates that the top spectrum was collected first and the lowest spectrum last. Note that successive spectra remain very similar. File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title FigS2_RawData
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Description Figure S2. µXRF maps of element distributions in unstained methacrylate-embedded thin mid-tubule sections of woodlouse hepatopancreas sampled at 02:00 hours (i.e. the beginning of ‘B’-cell restitution during darkness). A. Light micrograph of a transverse section. S5_sp_light1x10.tif B. Superimposed Cu, Fe and Zn µXRF maps of the section depicted in S1A; note the regular 1:1 distribution of ‘S’- (*) and ‘B’-cells (•) in the tubule wall. Prefix 5955 C. Expanded view µXRF maps for the region delineated by a broken-lined rectangle in 2E; note the hallmarks of Cu/Zn-containing ‘S’-cell cytoplasm ‘under’ the basal aspect of the ‘B’-cell (dotted line), the co-distribution of Cu and Zn (light blue) as well as partitioning of Cu (green) and Zn (dark blue) in the ‘S’-cell, and possibly some Zn-containing focal regions in the ‘B’-cell (broken arrows). Prefix 5956 File types included: tif - Image files (Light Micrographs) .dat : map data showing the stage positions in mm and windowed fluorescence counts. Column format: Horizontal position ; vertical position; time per point (ms); I0; It; Idrain; mca file. .edf file ESRF format file for program PyMCA containing the summed MCA files for each point in a map row by row. Binary data format .rgb file windowed detector counts for selected chemical elements of interest , column format: y index; x index; time; i0 it idrain; then elements as listed in first row.
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Title FigS3_RawData
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Description Figure S3. µXRF maps of element distributions in unstained methacrylate-embedded thin mid-tubule sections of woodlouse hepatopancreas sampled at 02:00 hours (i.e. the beginning of ‘B’-cell restitution during darkness). A. Light micrograph of a transverse section; note two spherical structures (arrows) in the lumen that might represent the newly shed apical cytoplasm (‘blebbing’) of two adjacent ‘B’-cells. S5_sp_light2.tif B. Superimposed Cu, Fe and Zn µXRF maps of the section depicted in S2A; note the Fe-rich ‘blebs’ in the lumen (solid arrows) corresponding with the structures labelled in S2A, as well as two more diffuse entities (broken arrows) possibly representing ‘older’ blebs that were not discernible in the micrograph. Sample Prefix 6076 File types included: tif - Image files (Light Micrographs) .dat : map data showing the stage positions in mm and windowed fluorescence counts. Column format: Horizontal position ; vertical position; time per point (ms); I0; It; Idrain; mca file. .edf file ESRF format file for program PyMCA containing the summed MCA files for each point in a map row by row. Binary data format .rgb file windowed detector counts for selected chemical elements of interest , column format: y index; x index; time; i0 it idrain; then elements as listed in first row.
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Title FigS4_RawData
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Description Figure S4. Radiation sensitivity of Cu assessed by collecting a sequential series of 5 XANES spectra from given regions in 3 different ‘S’-cells (panels A, B, C). Panel A Spectral Range 6099-6103 (n=5) Panel B Spectral Range 6085-89 (n=5) Panel C Spectral Range 6123-27 (n=5) The thin methacrylate-embedded sections were obtained from a woodlouse sampled at 02:00 hours. In each panel, the large down-pointing vertical arrow indicates that the top spectrum was collected first and the lowest spectrum last. Note that the small shoulder on the absorption edge at 8982.5 eV (broken arrows) is slightly eroded from the third acquired spectrum onwards (cf. Fig. 7). File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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Title FigS5_RawData
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Description Figure S5. Radiation sensitivity of Zn assessed by collecting a sequential series of 5 XANES spectra from given regions in 2 different ‘S’-cells (panels A and B). Panel A Spectral Range 6142-46 Panel B Spectral Range 6116-21 The thin methacrylate-embedded sections were obtained from a woodlouse sampled at 0200 hours. In each panel, the large down-pointing vertical arrow indicates that the top spectrum was collected first and the lowest spectrum last. Note that the absorption edge peak at ~9670 eV (broken arrows) is slightly eroded from the 2nd acquisition onwards (A) and from the 4th onwards (B). File type provided: XAS _dtc.dat - deadtime corrected (DTC) XAS files; column format: Monochromator angle : energy : time per point : I0 : It; Idrain : raw fluorescence counts channels 1-9: summed raw fluorescence counts: DTC flu counts channels 1-9: summed DTC fluorescence counts
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When using this data, please cite the original publication:

Kille P, Morgan AJ, Powell K, Mosselmans JFW, Hart D, Gunning P, Hayes A, Scarborough D, Mcdonald I, Charnock JM (2016) ‘Venus trapped, Mars transits’: Cu and Fe redox chemistry, cellular topography and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biology 6: 150270. http://dx.doi.org/10.1098/rsob.150270

Additionally, please cite the Dryad data package:

Kille P, Morgan AJ, Powell K, Mosselmans JFW, Hart D, Gunning P, Hayes A, Scarborough D, Mcdonald I, Charnock JM (2016) Data from: ‘Venus trapped, Mars transits’: Cu and Fe redox chemistry, cellular topography and in situ ligand binding in terrestrial isopod hepatopancreas. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.8734c
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