Data from: Assessment of intrathecal free light chain synthesis: comparison of different quantitative methods with the detection of oligoclonal free light chains by isoelectric focusing and affinity-mediated immunoblotting

Zeman D, Kušnierová P, Švagera Z, Všianský F, Byrtusová M, Hradílek P, Kurková B, Zapletalová O, Bartoš V

Date Published: November 17, 2016

DOI: http://dx.doi.org/10.5061/dryad.8tk26

 

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Title Raw data in Excel Table
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Title Analytical characteristics of the in house ELISA methods
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Description Analytical characteristics of the in house ELISA methods – Table Legend CoH – fKLC Freelite high control; CoL – fKLC Freelite low control; CSF1, CSF2, CSF3 – pooled CSF samples; S1, S2, S3 – pooled serum samples; 1/10, 1/100, 1/250 – pooled CSF dilutions. Pooled sera as well as Freelite controls were diluted 1/1000. SD – standard deviation; CV % – coefficient of variation. Flebogamma 40 mg/L was added to test maximal theoretical cross-reaction with light chains bound in the IgG. There is, however, no information available whether intravenous immunoglobulin preparations may contain traces of free light chains. Please note that in Method D, sample S1 was out of the calibration range of the fLLC assay. Estimated bias is only provided for method E since there is no internationally recognized standard for fLC; hence, Freelite controls were presumed to give the indicated concentrations only in conjunction with Freelite calibrators. Recovery assays: One of the pooled CSFs and sera was spiked with 10% (vol/vol) of either Bethyl purified monoclonal fLC or Freelite standards to obtain the theoretical concentration indicated (i.e., B16 corresponds to the final concentration of 16 ug/L in the sample). The expected concentration was then calculated as the final theoretical concentration + 0.9 * concentration measured in the non-spiked sample within the same analytical run. Recovery was calculated as 100 * (measured concentration – expected concentration)/expected concentration.
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Title FLC SPA preanalytics
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Description 11 CSF samples were divided in two aliquots; one was frozen to -30°C immediately upon receipt (A), the second was stored at +2 to +8°C for exactly 7 days and frozen thereafter. All samples were then analysed for free light chains within the same analytical run using the Freelite assay on SPA PLUS analyser.
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Title fKLC preanalytics
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Description 11 CSF samples were divided into two aliquots and frozen either immediately (A) or after 7 days at +2°C to +8°C (B). Only marginal, albeit statistically significant, decrease of fKLC concentrations has been found in (B).
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Title fLLC preanalytics
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Description 11 CSF samples were divided into two aliquots and frozen either immediately (A) or after 7 days at +2°C to +8°C (B). No changes of fLLC concentrations have been observed in (B).
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When using this data, please cite the original publication:

Zeman D, Kušnierová P, Švagera Z, Všianský F, Byrtusová M, Hradílek P, Kurková B, Zapletalová O, Bartoš V (2016) Assessment of intrathecal free light chain synthesis: comparison of different quantitative methods with the detection of oligoclonal free light chains by isoelectric focusing and affinity-mediated immunoblotting. PLOS ONE 11(11): e0166556. http://dx.doi.org/ 10.1371/journal.pone.0166556

Additionally, please cite the Dryad data package:

Zeman D, Kušnierová P, Švagera Z, Všianský F, Byrtusová M, Hradílek P, Kurková B, Zapletalová O, Bartoš V (2016) Data from: Assessment of intrathecal free light chain synthesis: comparison of different quantitative methods with the detection of oligoclonal free light chains by isoelectric focusing and affinity-mediated immunoblotting. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.8tk26
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