Data from: Sorting things out: assessing effects of unequal specimen biomass on DNA metabarcoding

Elbrecht V, Peinert B, Leese F

Date Published: July 31, 2017

DOI: https://doi.org/10.5061/dryad.8v528

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Title Metabarcoding pipeline and scripts
Downloaded 9 times
Description Full metabarcoding pipeline and R scripts to produce figures and statistical analysis in the paper. Includes intermediate scripts, always use the script with the latest version number.
Download 2 HiSeq biomass 160707.zip (74.42 Mb)
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Title Dereplicated and preprocessed metabarcoding data
Downloaded 6 times
Description These are the processed reads that were used for OTU clustering. Files are 10 samples (5 for each stream) amplified with 4 primer pairs (=40). The two sequencing lanes are differentiated with L001 and L002 but were merged into one file for each of the 40 sample in the analysis (only sequencing replicates). NOTE: If you want to access the raw sequencing data without any processing, please refer to the NCBI SRA accession numbers in the manuscript.
Download 6 dereplicate.zip (375.8 Mb)
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Title FastQC and run information
Downloaded 7 times
Description FastQC check and metadata for all 3 lanes run on the Illumina HiSeq (1,5 rapid runs v2, 250 bp PE).
Download FastQC.zip (8.946 Mb)
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When using this data, please cite the original publication:

Elbrecht V, Peinert B, Leese F (2017) Sorting things out: Assessing effects of unequal specimen biomass on DNA metabarcoding. Ecology and Evolution 7(17): 6918-6926. https://doi.org/10.1002/ece3.3192

Additionally, please cite the Dryad data package:

Elbrecht V, Peinert B, Leese F (2017) Data from: Sorting things out: assessing effects of unequal specimen biomass on DNA metabarcoding. Dryad Digital Repository. https://doi.org/10.5061/dryad.8v528
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