Data from: Reproductive interference and fecundity affect competitive interactions of sibling species with low mating barriers: experimental and theoretical evidence

Gebiola M, Kelly SE, Velten L, Zug R, Hammerstein P, Giorgini M, Hunter MS

Date Published: August 10, 2017

DOI: http://dx.doi.org/10.5061/dryad.8v739

 

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Title Longevity experiment
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Description We examined the relative longevity of parental species vs. hybrid progeny. We isolated 20 female pupae of EG, iES, cES and hybrids (produced by mating EG and cES wasps in both directions) in 1.2 ml vials, and at emergence we fed the adults with a small drop of honey, and kept them at a constant temperature of 27°C. Vials were checked daily, honey was added when necessary, and the date of female death was recorded.
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Title Fecundity experiment
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Description We compared fecundity of 3 longstanding laboratory colonies (1st exp) and of 2 longstanding lab colonies and colonies recently established from field collected individuals (2nd exp). Female wasps were individually paired for 24 h in small vials with a conspecific male. Females were then placed individually in arenas with leaf disks bearing about 80 whitefly nymphs. The leaf disks rested on a layer of agar in ventilated 35 mm agar Petri dishes. Females were transferred to new whitefly-infested arenas every other day until day eight. At day 4, we provided females with the opportunity to mate again to avoid the risk of sperm depletion by introducing a male into the arena for 24h. Arenas were kept at 27°C, 16L:8D, and 65% RH. After females were removed on day 8, arenas were incubated for approximately 10 more days. At the end of this period, we recorded the number of progeny pupae, a proxy for adult emergence, as a measure of fecundity.
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Title High Resolution Melting data
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Description For each cage of each generation, we randomly selected 24 females among those stored in ethanol at the end of each generation by pipetting without observation. The number of parental species and hybrid progeny per cage per generation as identified by the High Resolution Melting assay is presented here.
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Gebiola M, Kelly SE, Velten L, Zug R, Hammerstein P, Giorgini M, Hunter MS (2017) Data from: Reproductive interference and fecundity affect competitive interactions of sibling species with low mating barriers: experimental and theoretical evidence. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.8v739
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