Data from: TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis

Jha KN, Tripurani SK, Johnson GR

Date Published: April 5, 2017

DOI: http://dx.doi.org/10.5061/dryad.95s1k

 

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Title RNA-Seq data of WT and Tssk6 KO spermatids
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Description Spermatids were purified from three sets of adult wild type (WT) and Tssk6 knockout (KO), total RNA was isolated, RNA-Seq libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kit, and sequenced on Illumina HiSeq 2500. Quality of raw data was assessed and passed by FastQC and reads were mapped to the reference genome (Mus musculus_UCSC_mm10). Cufflinks (v2.2.1) was used to estimate transcript levels represented by FPKM (fragments per kilo bases per million mapped reads) and differential expression between samples was determined by Cuffdiff (v.2.2.1). Student’s t-test was performed to calculate the P values.
Download RNA Seq Data_Jha et al.xlsx (4.208 Mb)
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When using this data, please cite the original publication:

Jha KN, Tripurani SK, Johnson GR (2017) TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis. Journal of Cell Science 130(10): 1835-1844. http://dx.doi.org/10.1242/jcs.202721

Additionally, please cite the Dryad data package:

Jha KN, Tripurani SK, Johnson GR (2017) Data from: TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.95s1k
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