Data from: Fine-scale spatial ecology drives kin selection relatedness among cooperating amoebae

Smith J, Strassmann JE, Queller DC

Date Published: February 19, 2016

DOI: http://dx.doi.org/10.5061/dryad.983r5

 

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Title Data: Spatial distribution of D. discoideum in soil
Downloaded 2 times
Description Abundance of Dictyostelium discoideum isolates in small soil samples collected along transects. See Fortunato et al (2003) Mol Ecol 12:1031 and Gilbert et al (2012) Proc Roy Soc Lond B 279:2619.
Download data-soil-density.txt (3.087 Kb)
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Title Analysis: Spatial distribution of D. discoideum in soil
Downloaded 8 times
Description Analyze and visualize spatial distribution of Dictyostelium discoideum amoebae among soil samples. Script for use with R environment for statistical computing.
Download analysis-soil-density.R (12.64 Kb)
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Title Data: D. discoideum plaque growth
Downloaded 5 times
Description Growth of Dictyostelium discoideum plaques on bacterial lawns
Download data-plaque-growth.txt (3.303 Kb)
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Title Analysis: D. discoideum plaque growth
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Description Analyze and visualize growth of Dictysotelium discoideum plaques on Klebsiella lawns. Script for use with R environment for statistical computing.
Download analysis-plaque-growth.R (22.55 Kb)
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Title Data: Transect of D. discoideum fruiting bodies across two intersecting plaques
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Description Distribution of genotypes along transect of D. discoideum fruiting bodies across two intersecting plaques.
Download data-plaque-transect.txt (7.302 Kb)
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Title Analysis: Transect of D. discoideum fruiting bodies across two intersecting plaques
Downloaded 6 times
Description Analyze and visualize distribution of genotypes along transect of Dictyostelium discoideum fruiting bodies across two intersecting plaques. Script for use with R environment for statistical computing.
Download analysis-plaque-transect.R (9.692 Kb)
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Title Analysis: D. discoideum flow cytometry
Downloaded 7 times
Description Parse flow cytometry .fcs files to determine number and proportion of fluorescent/nonfluorescent Dictyostelium discoideum cells. Script for use with R environment for statistical computing.
Download count_dicty_cells.R (4.088 Kb)
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Title Data: D. discoideum fruiting bodies sampled from petri plate
Downloaded 1 time
Description Genotype composition of individual Dictyostelium discoideum fruiting bodies sampled from across surface of petri plate. Generated by analysis of flow cytometry data.
Download data-relatedness.txt (139.1 Kb)
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Title Analysis: D. discoideum fruiting bodies sampled from petri plate
Downloaded 9 times
Description Analyze and visualize distribution of Dictyostelium discoideum genotypes in individual fruiting bodies sampled from across surface of petri plates. Script for use with R environment for statistical computing.
Download analysis-relatedness.R (32.44 Kb)
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Title Data: Germination of D. discoideum spores on bacterial lawns
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Description Number of plaques observed on bacterial lawns inoculated with known number of Dictyostelium discoideum spores.
Download data-germination.txt (1.931 Kb)
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Title Data: D. discoideum inocula
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Description Genotype composition of Dictyostelium discoideum samples used to inoculate bacterial lawns. Generated by analysis of flow cytometry data.
Download data-inocula.txt (10.1 Kb)
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Title Data: Flow cytometry of fruiting bodies from transect across plaques
Downloaded 5 times
Description Raw flow cytometry data (FCS format) from Dictyostelium discoideum fruiting bodies sampled along transect crossing two plaques.
Download fcs-files-transect.zip (48.48 Mb)
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Title Data: Flow cytometry of D. discoideum inocula and all-RFP controls
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Description Raw flow cytometry data (FCS format) of Dictyostelium discoideum spores (1) inoculated onto bacterial lawns and (2) sampled from fruiting bodies on plates only inoculated with RFP-labelled amoebae.
Download fcs-files-controls.zip (994.3 Mb)
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Title Data: Flow cytometry of D. discoideum fruiting bodies sampled from across plate
Downloaded 5 times
Description Raw flow cytometry data (FCS format) of Dictyostelium discoideum spores sampled from fruiting bodies on plates inoculated with mix of wild type and RFP-labelled amoebae.
Download fcs-files-relatedness.zip (904.6 Mb)
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Title Data: D. discoideum microscopy
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Description Individual and composited images of Dictyostelium discoideum fruiting bodies.
Download dicty-images.zip (317.9 Mb)
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When using this data, please cite the original publication:

Smith J, Strassmann JE, Queller DC (2016) Fine-scale spatial ecology drives kin selection relatedness among cooperating amoebae. Evolution 70(4): 848–859. http://dx.doi.org/10.1111/evo.12895

Additionally, please cite the Dryad data package:

Smith J, Strassmann JE, Queller DC (2016) Data from: Fine-scale spatial ecology drives kin selection relatedness among cooperating amoebae. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.983r5
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