Data from: Comparative landscape genetics of two river frog species occurring at different elevations on Mount Kilimanjaro

Zancolli G, Rödel M, Steffan-Dewenter I, Storfer A

Date Published: September 17, 2014

DOI: http://dx.doi.org/10.5061/dryad.9pb01

 

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Title AFLP dataset
Downloaded 45 times
Description Amietia wittei and Amietia angolensis tissue samples were collected from sites on the southern slope of Mount Kilimanjaro, Tanzania, in 2011. Total genomic DNA was extracted with the Roche High Pure PCR Template Preparation Kit according to the manufacturer's protocol, except for an extra step after the digestion by proteinase K aiming to discard residual pigments. DNA was digested and fragments were amplified using six primer pair combinations using a modified version of Vos et al. (1995) (Appendix S1, Supporting Information). AFLP fragments were separated in an ABI 3130XL automatic capillary sequencer (Applied Biosystems) with an internal GeneScan 500 ROX size standard at the Department of Human Genetics, Biozentrum, University of Würzburg. AFLP peaks were determined using GeneMapper 4.1 (Applied Biosystems) and the final binary matrices were obtained following thw semi-automated scoring procedure described in Whitlock et al. (2008). The data include presence (1), absence (0), unscored (-1) AFLP fragments (in column) for each study site (rows) for Amietia wittei and Amietia angolensis separately.
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Title LCP distance data
Downloaded 33 times
Description Landscape distances and variables included in each least-cost path (LCP) between study sites of Amietia wittei and Amietia angolensis calculated in ArcGIS 10 (ESRI). For each LCP model between pairs of sites (rows) the following variables (columns) were calculated: topography-corrected route lengths (distance); weighted-average values of compound topographic index (cti), roughness (rough) and slope; proportions of cropland, homegarden, human settlements, erica shrubs, forest, disturbed forest, reforestation and grassland. A 30 x 30 m digital elevation model (DEM) produced by J.A. Ong'injo, C. Lambrechts and A. Help was use for calculating resistance surfaces. Stream network was delineated using the "flow accumulation" tool in ArcGIS 10 (ESRI). The land cover surface was modified from the original produced by T. Appelhans and T. Nauss (unpubl.). Straight-line distances (Euclidean) are also reported and used as null models.
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Title Amietia 16S aligned sequences
Downloaded 4 times
Description Aligned sequences of the 16S mitochondrial rRNA gene from 14 randomly selected individuals (GenBank accession numbers: KJ469265-KJ469278) of Amietia spp on Mt. Kilimanjaro (Tanzania).
Download Amietia_16S_aligned.txt (7.151 Kb)
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When using this data, please cite the original publication:

Zancolli G, Rödel M, Steffan-Dewenter I, Storfer A (2014) Comparative landscape genetics of two river frog species occurring at different elevations on Mount Kilimanjaro. Molecular Ecology 23(20): 4989-5002. http://dx.doi.org/10.1111/mec.12921

Additionally, please cite the Dryad data package:

Zancolli G, Rödel M, Steffan-Dewenter I, Storfer A (2014) Data from: Comparative landscape genetics of two river frog species occurring at different elevations on Mount Kilimanjaro. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.9pb01
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