Data from: Genomic DNA transposition induced by human PGBD5

Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A

Date Published: September 28, 2015

DOI: http://dx.doi.org/10.5061/dryad.b2hc1

 

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Title Reads from HEK293-GFP-PBwt mapped to hg19+pb-ef1_neo.
Downloaded 3 times
Description Reads obtained from sequencing FLEA PCR products to amplify transposon reporter insertions in sample HEK293-GFP-PBwt, mapped to a hybrid genome consisting of hg19 and the reporter plasmid sequence. Reads were mapped with bwa-mem using default parameters.
Download pcr1_HEK293-GFP-PBwt.vs.hg19_pbneo.mem.bam (100.4 Mb)
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Title Reads from HEK293-GFP-PBmut mapped to hg19+pb-ef1-neo
Downloaded 2 times
Description Reads obtained from sequencing FLEA PCR products to amplify transposon reporter insertions in sample HEK293-GFP-PBmut, filtered, quality trimmed and mapped to a hybrid genome consisting of hg19 and the reporter plasmid sequence. Reads were mapped with bwa-mem using default parameters.
Download pcr2_HEK293-GFP-PBmut.vs.hg19_pbneo.mem.bam (213.0 Mb)
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Title Reads from HEK293-PGBD5-PBwt mapped to hg19+pb-ef1-neo
Downloaded 2 times
Description Reads obtained from sequencing FLEA PCR products to amplify transposon reporter insertions in sample HEK293-PGBD5-PBwt, filtered, quality trimmed and mapped to a hybrid genome consisting of hg19 and the reporter plasmid sequence. Reads were mapped with bwa-mem using default parameters.
Download pcr3_HEK293-PGBD5-PBwt.vs.hg19_pbneo.mem.bam (143.4 Mb)
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Title Reads from HEK293-PGBD5-PBmut mapped to hg19+pb-ef1-neo
Downloaded 4 times
Description Reads obtained from sequencing FLEA PCR products to amplify transposon reporter insertions in sample HEK293-PGBD5-PBmut, filtered, quality trimmed and mapped to a hybrid genome consisting of hg19 and the reporter plasmid sequence. Reads were mapped with bwa-mem using default parameters.
Download pcr4_HEK293-PGBD5-PBmut.vs.hg19_pbneo.mem.bam (437.7 Mb)
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Title Summary table of DNA transposon integration loci
Downloaded 5 times
Description Spreadsheet listing human genome and transposon coordinate positions of transposon integrations, integration type (ITR transposition vs non-ITR transposition), and DNA sequences surrounding the genomic integration sites.
Download summary_insertion_sites_pub.xlsx (70.50 Kb)
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Title Transposon reporter plasmid sequence: pb-ef1-neo
Downloaded 8 times
Description Plasmid sequence of NEO reporter flanked by PiggyBac ITRs. Reads from FLEA PCR were mapped to a hybrid genome consisting of this sequence and the hg19 human genome reference.
Download pb-ef1-neo_seq.fa (7.046 Kb)
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When using this data, please cite the original publication:

Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A (2015) Genomic DNA transposition induced by human PGBD5. eLife 4:e10565. http://dx.doi.org/10.7554/eLife.10565

Additionally, please cite the Dryad data package:

Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A (2015) Data from: Genomic DNA transposition induced by human PGBD5. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.b2hc1
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