Show simple item record Shehzad, Wasim McCarthy, Thomas Michael Pompanon, François Purevjav, Lkhagvajav Coissac, Eric Riaz, Tiayyba Taberlet, Pierre
dc.coverage.spatial Tost Mountains
dc.coverage.spatial South Gobi Mongolia
dc.coverage.temporal Present 2012-11-07T18:17:48Z 2012-11-07T18:17:48Z 2012-02-29
dc.identifier doi:10.5061/dryad.bj376f61
dc.identifier.citation Shehzad W, McCarthy TM, Pompanon F, Purevjav L, Coissac E, Riaz T, Taberlet P (2012) Prey preference of snow leopard (Panthera uncia) in South Gobi, Mongolia. PLoS ONE, 7(2): e32104.
dc.description Accurate information about the diet of large carnivores that are elusive and inhabit inaccessible terrain, is required to properly design conservation strategies. Predation on livestock and retaliatory killing of predators have become serious issues throughout the range of the snow leopard. Several feeding ecology studies of snow leopards have been conducted using classical approaches. These techniques have inherent limitations in their ability to properly identify both snow leopard feces and prey taxa. To examine the frequency of livestock prey and nearly-threatened argali in the diet of the snow leopard, we employed the recently developed DNA-based diet approach to study a snow leopard population located in the Tost Mountains, South Gobi, Mongolia. After DNA was extracted from the feces, a region of ~100 bp long from mitochondrial 12S rRNA gene was amplified, making use of universal primers for vertebrates and a blocking oligonucleotide specific to snow leopard DNA. The amplicons were then sequenced using a next-generation sequencing platform. We observed a total of five different prey items from 81 fecal samples. Siberian ibex predominated the diet (in 70.4 % of the feces), followed by domestic goat (17.3 %) and argali sheep (8.6 %). The major part of the diet was comprised of large ungulates (in 98.8 % of the feces) including wild ungulates (79 %) and domestic livestock (19.7 %). The findings of the present study will help to understand the feeding ecology of the snow leopard, as well as to address the conservation and management issues pertaining to this wild cat.
dc.relation.haspart doi:10.5061/dryad.bj376f61/4
dc.relation.haspart doi:10.5061/dryad.bj376f61/5
dc.relation.haspart doi:10.5061/dryad.bj376f61/6
dc.relation.haspart doi:10.5061/dryad.bj376f61/7
dc.relation.haspart doi:10.5061/dryad.bj376f61/8
dc.relation.haspart doi:10.5061/dryad.bj376f61/9
dc.relation.isreferencedby doi:10.1371/journal.pone.0032104
dc.relation.isreferencedby PMID:22393381
dc.subject blocking oligonucleotide
dc.subject diet analysis
dc.subject DNA metabarcoding
dc.subject next generation sequencing
dc.subject Panthera uncia.
dc.title Data from: Prey preference of snow leopard (Panthera uncia) in South Gobi, Mongolia
dc.type Article
dwc.ScientificName Panthera uncia
dwc.ScientificName Capra hircus
dwc.ScientificName Capra sibirica
dwc.ScientificName Ovis aries
dwc.ScientificName Ovis ammon
dwc.ScientificName Alectoris Chukar
dc.contributor.correspondingAuthor Taberlet, Pierre
prism.publicationName PLoS ONE
dryad.dansTransferDate 2017-09-26T12:11:10.844-0400

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